Detailed information of TA system TA169409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4538391..4539022
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5C78_RS21895
• Protein ID: WP_075861597.1
• Coordinates: 4538747..4539022 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: H5C78_RS21890
• Protein ID: WP_000593557.1
• Coordinates: 4538391..4538750 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS21865	4534523..4535467	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
H5C78_RS21870	4535464..4536297	+	834	WP_021539320.1	nickel ABC transporter permease subunit NikC	-
H5C78_RS21875	4536297..4537061	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
H5C78_RS21880	4537058..4537864	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
H5C78_RS21885	4537870..4538271	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
H5C78_RS21890	4538391..4538750	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5C78_RS21895	4538747..4539022	-	276	WP_075861597.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5C78_RS21900	4539095..4540219	-	1125	WP_001318088.1	ABC transporter permease	-
H5C78_RS21905	4540219..4542954	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
H5C78_RS21910	4542951..4544018	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4505909..4542954	37045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10137.82 Da
• Isoelectric Point: 10.6128

>T169409 WP_075861597.1 NZ_CP059917:c4539022-4538747 [Escherichia coli]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T169409 NZ_CP076470:2582732-2583028 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT169409 WP_000593557.1 NZ_CP059917:c4538750-4538391 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT169409 NZ_CP076470:2583030-2583425 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: L4IZA1