Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3842565..3843219 Replicon chromosome
Accession NZ_CP059917
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag H5C78_RS18425 Protein ID WP_000244781.1
Coordinates 3842565..3842972 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H5C78_RS18430 Protein ID WP_000354046.1
Coordinates 3842953..3843219 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS18405 3838522..3840255 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
H5C78_RS18410 3840261..3840971 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H5C78_RS18415 3840996..3841892 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H5C78_RS18420 3842004..3842525 + 522 WP_001055874.1 flavodoxin FldB -
H5C78_RS18425 3842565..3842972 - 408 WP_000244781.1 protein YgfX Toxin
H5C78_RS18430 3842953..3843219 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H5C78_RS18435 3843472..3844452 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
H5C78_RS18440 3844529..3845188 - 660 WP_000250269.1 hemolysin III family protein -
H5C78_RS18445 3845352..3845663 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
H5C78_RS18450 3845708..3847141 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T169405 WP_000244781.1 NZ_CP059917:c3842972-3842565 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T169405 NZ_CP076470:c1398649-1398476 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT169405 WP_000354046.1 NZ_CP059917:c3843219-3842953 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT169405 NZ_CP076470:c1398973-1398677 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References