Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3391920..3392545 Replicon chromosome
Accession NZ_CP059917
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag H5C78_RS16400 Protein ID WP_000911329.1
Coordinates 3391920..3392318 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag H5C78_RS16405 Protein ID WP_000450524.1
Coordinates 3392318..3392545 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS16380 3387827..3388027 + 201 WP_000383836.1 YpfN family protein -
H5C78_RS16385 3388108..3388806 - 699 WP_025491901.1 esterase -
H5C78_RS16390 3388880..3390895 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
H5C78_RS16395 3390910..3391773 - 864 WP_001267498.1 neutral zinc metallopeptidase -
H5C78_RS16400 3391920..3392318 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
H5C78_RS16405 3392318..3392545 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
H5C78_RS16410 3392702..3393415 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
H5C78_RS16415 3393628..3394662 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
H5C78_RS16420 3394679..3395557 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
H5C78_RS16425 3395703..3396275 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
H5C78_RS16430 3396275..3396745 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T169404 WP_000911329.1 NZ_CP059917:c3392318-3391920 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T169404 NZ_CP076470:c1302865-1302515 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT169404 WP_000450524.1 NZ_CP059917:c3392545-3392318 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT169404 NZ_CP076470:c1303110-1302859 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References