Detailed information of TA system TA169394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2372190..2373772
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0J2BJD7
• Locus tag: H5C78_RS11410
• Protein ID: WP_001125469.1
• Coordinates: 2372190..2373506 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H5C78_RS11415
• Protein ID: WP_001296726.1
• Coordinates: 2373506..2373772 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS11395	2369579..2370289	-	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-
H5C78_RS11400	2370651..2371214	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
H5C78_RS11405	2371648..2371914	-	267	Protein_2229	helix-turn-helix transcriptional regulator	-
H5C78_RS11410	2372190..2373506	-	1317	WP_001125469.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
H5C78_RS11415	2373506..2373772	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H5C78_RS11420	2373965..2375854	-	1890	Protein_2232	autotransporter-associated beta strand	-
H5C78_RS11425	2376004..2377596	-	1593	WP_000113152.1	autoinducer-2 kinase	-
H5C78_RS11430	2377675..2378628	-	954	WP_182917273.1	transcriptional regulator LsrR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49275.91 Da
• Isoelectric Point: 8.8783

>T169394 WP_001125469.1 NZ_CP059917:c2373506-2372190 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY

Nucleotide

• Length: 1317 bp

>T169394 NZ_CP076454:c98155-97673 [Klebsiella pneumoniae]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACACAACATTGCTGAGTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAATCACAGAACGGGCATTTCAAGAGTTTTCGTCGTCTGTGCTGAAAACACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCTGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTAGGAGCTGCGCTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT169394 WP_001296726.1 NZ_CP059917:c2373772-2373506 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT169394 NZ_CP076454:c98424-98146 [Klebsiella pneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAATCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAGCCGCAAATCGCCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2BJD7