Detailed information of TA system TA169388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1927081..1927865
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5C78_RS09230
• Protein ID: WP_000613624.1
• Coordinates: 1927081..1927575 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: H5C78_RS09235
• Protein ID: WP_001110446.1
• Coordinates: 1927572..1927865 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS09210	1922274..1923371	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
H5C78_RS09215	1923371..1924312	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
H5C78_RS09220	1924378..1926021	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
H5C78_RS09225	1926033..1926986	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
H5C78_RS09230	1927081..1927575	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
H5C78_RS09235	1927572..1927865	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
H5C78_RS09240	1927999..1931184	-	3186	WP_000827395.1	ribonuclease E	-
H5C78_RS09245	1931757..1932716	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T169388 WP_000613624.1 NZ_CP059917:c1927575-1927081 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T169388 NZ_CP076453:4105926-4106144 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT169388 WP_001110446.1 NZ_CP059917:c1927865-1927572 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169388 NZ_CP076453:4105525-4105899 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829G775