Detailed information of TA system TA169384

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 947779..948577
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H5C78_RS04470
• Protein ID: WP_000854737.1
• Coordinates: 947779..948156 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: H5C78_RS04475
• Protein ID: WP_001285481.1
• Coordinates: 948203..948577 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS04445	943813..944104	+	292	Protein_861	transposase	-
H5C78_RS04450	944101..944451	+	351	WP_000624707.1	IS66 family insertion sequence element accessory protein TnpB	-
H5C78_RS04455	944482..946075	+	1594	Protein_863	IS66 family transposase	-
H5C78_RS04460	946223..947211	+	989	Protein_864	IS630 family transposase	-
H5C78_RS04465	947290..947782	-	493	Protein_865	hypothetical protein	-
H5C78_RS04470	947779..948156	-	378	WP_000854737.1	TA system toxin CbtA family protein	Toxin
H5C78_RS04475	948203..948577	-	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H5C78_RS04480	948627..949271	-	645	WP_000086759.1	hypothetical protein	-
H5C78_RS04485	949290..949511	-	222	WP_000692315.1	DUF987 domain-containing protein	-
H5C78_RS04490	949574..950050	-	477	WP_041498384.1	RadC family protein	-
H5C78_RS04495	950066..950539	-	474	WP_001313575.1	antirestriction protein	-
H5C78_RS04500	950633..950878	-	246	WP_001164966.1	hypothetical protein	-
H5C78_RS04505	950878..951699	-	822	WP_001234359.1	DUF945 domain-containing protein	-
H5C78_RS04510	951799..952032	-	234	WP_001213776.1	DUF905 family protein	-
H5C78_RS04515	952150..952602	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14240.26 Da
• Isoelectric Point: 7.8051

>T169384 WP_000854737.1 NZ_CP059917:c948156-947779 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T169384 NZ_CP076453:721278-721763 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT169384 WP_001285481.1 NZ_CP059917:c948577-948203 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT169384 NZ_CP076453:720988-721281 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0P0SQV8