Detailed information of TA system TA169383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 757795..758219
• Replicon: chromosome
• Accession: NZ_CP059917
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5C78_RS03595
• Protein ID: WP_001306201.1
• Coordinates: 758031..758219 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0J2BBI0
• Locus tag: H5C78_RS03590
• Protein ID: WP_000125563.1
• Coordinates: 757795..758028 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS03565	752868..753155	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
H5C78_RS03570	753214..754545	+	1332	WP_001183198.1	MFS transporter	-
H5C78_RS03575	754653..755183	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
H5C78_RS03580	755176..757038	+	1863	WP_000377115.1	glutathione-regulated potassium-efflux system protein KefC	-
H5C78_RS03585	757230..757709	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
H5C78_RS03590	757795..758028	+	234	WP_000125563.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H5C78_RS03595	758031..758219	+	189	WP_001306201.1	CcdB family protein	Toxin
H5C78_RS03600	758216..758332	+	117	WP_000148634.1	CcdB family protein	-
H5C78_RS03605	758329..759177	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
H5C78_RS03610	759184..759561	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
H5C78_RS03615	759564..760385	-	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
H5C78_RS03620	760382..761371	-	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
H5C78_RS03625	761371..762657	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T169383 WP_001306201.1 NZ_CP059917:758031-758219 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T169383 NZ_CP076453:358222-358569 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.58 Da
• Isoelectric Point: 5.8062

>AT169383 WP_000125563.1 NZ_CP059917:757795-758028 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAAIRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT169383 NZ_CP076453:358569-358868 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGCCCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J2BBI0