Detailed information of TA system TA169379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 89422..90023
• Replicon: plasmid p3
• Accession: NZ_CP059910
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9Z1Q0
• Locus tag: H5C80_RS27290
• Protein ID: WP_001216047.1
• Coordinates: 89422..89802 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: H5C80_RS27295
• Protein ID: WP_001190712.1
• Coordinates: 89802..90023 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS27265	84862..86346	-	1485	WP_032163774.1	hypothetical protein	-
H5C80_RS27270	86346..87539	-	1194	WP_000219604.1	terminase	-
H5C80_RS27275	87626..88078	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
H5C80_RS27280	88167..89210	-	1044	WP_000648827.1	DUF968 domain-containing protein	-
H5C80_RS27285	89238..89417	-	180	WP_032163776.1	hypothetical protein	-
H5C80_RS27290	89422..89802	-	381	WP_001216047.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H5C80_RS27295	89802..90023	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5C80_RS27300	90096..90485	-	390	WP_000506726.1	DNA repair protein	-
H5C80_RS27305	90609..90860	-	252	WP_001283837.1	DNA polymerase III subunit theta	-
H5C80_RS27310	91034..91243	+	210	WP_001344848.1	hypothetical protein	-
H5C80_RS27315	91228..91512	-	285	WP_001142394.1	hypothetical protein	-
H5C80_RS27320	91496..92434	-	939	WP_077767319.1	hypothetical protein	-
H5C80_RS27325	92416..92790	-	375	WP_000988658.1	hypothetical protein	-
H5C80_RS27330	92797..93090	-	294	WP_000269003.1	hypothetical protein	-
H5C80_RS27335	93269..93502	-	234	WP_000517420.1	hypothetical protein	-
H5C80_RS27340	93579..93839	-	261	WP_001554875.1	hypothetical protein	-
H5C80_RS27345	93836..94711	-	876	WP_060588826.1	DUF551 domain-containing protein	-
H5C80_RS27350	94708..94917	-	210	WP_000951706.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99361	99361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T169379 WP_001216047.1 NZ_CP059910:c89802-89422 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T169379 NZ_CP076449:2085181-2085435 [Aggregatibacter actinomycetemcomitans]
ATGATTTTAGCTTGGACGGAAACCGCCTGGGAAGATTATCTTTATTGGCAGCAAGTTGATAAAAAGACGTTGTTGCGCAT
TAATAAACTTATCCAAAATATTACCAGATCACCTTTCGAAGGCTTAGGCAATCCTGAACCTTTAAAACATCAGTTATCCG
GGTTTTGGTCTAGAAGAATAGATAAAGAGCATCGTCTTGTATACCAAGTATCGGATAGCCATTTAACGATTATTCAATGC
CGCTATCACTACTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT169379 WP_001190712.1 NZ_CP059910:c90023-89802 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT169379 NZ_CP076449:2084933-2085184 [Aggregatibacter actinomycetemcomitans]
ATGAATGTAATAAGTTATTCTGCGTTTCGTGCAGAATTGGCAACCACACTTGATCAGGTGGTAGCAGATCATAGCCCAGT
CATGATTACCAGACAAAATGGCAAACACGCTGTTGTAATGAGTTTAGAAGATTTCGCAGCTTACGAAGAAACGGCTTATT
TATTACGCAGCCCCAAAAATAGAGAGCGTTTATTAGCCTCTATTGACCAGCTCAATTCAGGTAAAATCATCGAACGGGAA
CTCCAGGAATGA

Structures

Toxin

• AlphaFold DB: U9Z1Q0

Antitoxin

• AlphaFold DB: A0A829CJB6