Detailed information of TA system TA169373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 110912..111537
• Replicon: plasmid p1
• Accession: NZ_CP059908
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5C80_RS25690
• Protein ID: WP_000911313.1
• Coordinates: 111139..111537 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H5C80_RS25685
• Protein ID: WP_000450520.1
• Coordinates: 110912..111139 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS25685	110912..111139	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H5C80_RS25690	111139..111537	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H5C80_RS25695	111546..113753	-	2208	WP_021526192.1	type IV conjugative transfer system coupling protein TraD	-
H5C80_RS25700	114006..114737	-	732	WP_023565183.1	complement resistance protein TraT	-
H5C80_RS25705	114769..115266	-	498	WP_000605858.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucB / iucA	1..193291	193291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T169373 WP_000911313.1 NZ_CP059908:111139-111537 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T169373 NZ_CP076449:c1806054-1805761 [Aggregatibacter actinomycetemcomitans]
ATGACAGTAACAACCCGAGAATTTGATGCAGCGCGTTATTTGAAAAATGAAAAAATGATGGCAATGTATCTTGAAACAGT
TTTAGAAGAAGGCACAACGGCTGAGTTTATACAAGCTCTGAATACGGTCGCAAGAGCAAAGGGCATGTCGGAACTGGCGG
AAAAAACCGGCATCGGACGTGAAAGTTTGTACAAAACCCTATCAAGTGAAAAACCACGTTTTGACACGATTCTAAAAATT
ATTTCAGCATTGGGGCTTAGCCTTTCTATTAATGCTAAAGATGCAGTAGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT169373 WP_000450520.1 NZ_CP059908:110912-111139 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT169373 NZ_CP076449:c1806358-1806056 [Aggregatibacter actinomycetemcomitans]
ATGTATATAATTGAGGAAACGGAGGAATTCTCCAACTGGTTAGAGAATCTAACCGACGAACTTGCGATTGTGAATATTGT
TGCTCGAATCGAAAGGGCAAAGCTCGACAATTTCGGTGATCATAAATCGGTGGGTAATGGCGTATATGAAATGCGAATTA
CAAAAGGGAAAGGCTATAGGATCTACTATGCCAGAAAAGGGGGTATTACTTATCTATTGATTCTTGGTGGCGATAAGTCA
AGCCAAGAACAAGATATTCAGAATGCAAAAGCACTATGGCAACAGATTAAAAACGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2