Detailed information of TA system TA169366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4946154..4946578
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5C80_RS24310
• Protein ID: WP_001306201.1
• Coordinates: 4946154..4946342 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0J2BBI0
• Locus tag: H5C80_RS24315
• Protein ID: WP_000125563.1
• Coordinates: 4946345..4946578 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS24280	4941716..4943002	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
H5C80_RS24285	4943002..4943991	+	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
H5C80_RS24290	4943988..4944809	+	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
H5C80_RS24295	4944812..4945189	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
H5C80_RS24300	4945196..4946044	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
H5C80_RS24305	4946041..4946157	-	117	WP_000148634.1	CcdB family protein	-
H5C80_RS24310	4946154..4946342	-	189	WP_001306201.1	CcdB family protein	Toxin
H5C80_RS24315	4946345..4946578	-	234	WP_000125563.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H5C80_RS24320	4946664..4947143	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
H5C80_RS24325	4947335..4949197	-	1863	WP_000377115.1	glutathione-regulated potassium-efflux system protein KefC	-
H5C80_RS24330	4949190..4949720	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
H5C80_RS24335	4949828..4951159	-	1332	WP_001183198.1	MFS transporter	-
H5C80_RS24340	4951218..4951505	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T169366 WP_001306201.1 NZ_CP059907:c4946342-4946154 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T169366 NZ_CP076443:1417321-1417593 [Gemella sp. zg-570]
ATGTATCATATCAAGTACACCAATAAATTCAAAAAAAGTTATAAACTAATGAAGAAACGAGGCTATGATCTTAGTTTATT
AGAGGATGTCATCACAAAATTAAGTAAAGGCATTCCTCTCGAAGAAAAACATAGGAATCACATGTTAACAGGTAATTTTG
CTGGATATCATGAATGTCATATCAAACCAGATTGGCTTTTAATTTATTTAATAGAAAATGATATTCTAACCTTAACTATG
ATCGATACAGGGTCGCATTCAGATTTATTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.58 Da
• Isoelectric Point: 5.8062

>AT169366 WP_000125563.1 NZ_CP059907:c4946578-4946345 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAAIRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT169366 NZ_CP076443:1417046-1417321 [Gemella sp. zg-570]
ATGGCTACAAGTCCAACTCAAATTAGAATCGATTCGACTGTTAAAGAAGATGCCAACGCATTATTTTCGAGACTTGGCTT
AGATATGTCTAGTGCAGTGAATATGTTCTTACGTCAATGCGTTCTACGTGGAGGTTTACCTTTTACAGTAGAACTTCCAA
AGTATAATCAAGAAACGTTAGATGCTATGGAAGAAGCAAGAAAAATTTCTAAAGACCCAAATGTACCAAGTTATTCATCT
GTTAAAGATTTATTTAAAGCATTGGATGAAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0J2BBI0