Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4484483..4485101 Replicon chromosome
Accession NZ_CP059907
Organism Escherichia coli strain 181.1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H5C80_RS22170 Protein ID WP_001291435.1
Coordinates 4484883..4485101 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H5C80_RS22165 Protein ID WP_000344800.1
Coordinates 4484483..4484857 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C80_RS22155 4479572..4480765 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H5C80_RS22160 4480788..4483937 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H5C80_RS22165 4484483..4484857 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H5C80_RS22170 4484883..4485101 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H5C80_RS22175 4485273..4485824 + 552 WP_000102564.1 maltose O-acetyltransferase -
H5C80_RS22180 4485940..4486410 + 471 WP_000136192.1 YlaC family protein -
H5C80_RS22185 4486574..4488124 + 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H5C80_RS22190 4488166..4488519 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H5C80_RS22200 4488898..4489209 + 312 WP_000409911.1 MGMT family protein -
H5C80_RS22205 4489240..4489812 - 573 WP_000779821.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169364 WP_001291435.1 NZ_CP059907:4484883-4485101 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T169364 NZ_CP076414:490252-490602 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT169364 WP_000344800.1 NZ_CP059907:4484483-4484857 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT169364 NZ_CP076414:489965-490246 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References