Detailed information of TA system TA169362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4070676..4071381
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H5C80_RS20080
• Protein ID: WP_000539521.1
• Coordinates: 4070676..4071062 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H5C80_RS20085
• Protein ID: WP_001280945.1
• Coordinates: 4071052..4071381 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS20060	4066680..4067306	+	627	WP_001295292.1	glutathione S-transferase GstB	-
H5C80_RS20065	4067303..4068418	-	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
H5C80_RS20070	4068529..4068912	-	384	WP_000497137.1	biofilm formation regulator BssR	-
H5C80_RS20075	4069125..4070450	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H5C80_RS20080	4070676..4071062	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5C80_RS20085	4071052..4071381	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H5C80_RS20090	4071451..4072779	-	1329	WP_000086919.1	GGDEF domain-containing protein	-
H5C80_RS20095	4072787..4075135	-	2349	Protein_3924	CHASE9 sensor domain-containing protein	-
H5C80_RS20100	4075313..4076224	-	912	WP_182928158.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T169362 WP_000539521.1 NZ_CP059907:4070676-4071062 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T169362 NZ_CP076412:1711821-1712723 [Ralstonia wenshanensis]
ATGAGCGCGCAGGCTGTCATGTCTTCCCATCCGTTCCGCGTTGCCGACGCGCTTGCCGTGCTCGGCGCCGGGTTTGCCGA
ACGCGCCGTCGCCATCAACGCGCACGGCGACCGCATCGGCTATCGGCAATGGGGCGAGCGCGGGCCGGTGGTGGTGCTGT
TGCACGGCATCAGCTCCAGCGCTGCATCATGGCTGCCTTGCGCGCAGGTTCTTTCGCGCAACATGCGTGTGCTGGCATGG
GATGCGCCGGGCTACGGCAATTCCACGCCGCTTGAGCAGGCTACGCCGAGCGCCGCCGACTACGCGGTGCGGCTGCAGGC
GTGGCTTGCCGCGCTGGATGTGACGCCCGATGTGATCGTCGGTCACTCGCTGGGCGCGCTGATGGCTTCGGCGTATGTGG
CCGCCGCGCCAGCGGCGATGCAGCCGAAGTGCCTCTTGCTTCTGAACCCTGCACAGGGCTACGGCCAGCCGGGGCAGGAA
GACAAGTCGCGCGCCGTGCAAGCGCAACGTCTTTCAACGCTGGACCAGCTTGGCATCGCCGGCATGGCGGAGTCGCGCCA
CGCGCACCTGCTGCGCCCGAATGCCACCGATGAAGAGCGTGCCTGGGTGCGCTGGAACATGAAGCGCCTCAACGATGGCG
GTTACCGCCAGGCCGTGGCGATGCTCAGCGGCGACGACATCGGCGCCTATCTGCGCAAACGCCCCGCATCGACGCCGGCA
CAAGTCGCTTGCGGCGATGTGGATGGCATCACGCCGCCGCAAGGCTGCGAAGCGCTGGCCAAGACGTTCGGTCTGCCTTT
TGCGCTAGTACCGGCTGCGGGGCATGCCTCCTATATCGATGCGCCTGACGCTGTCGCAGGCTGGATCGAACACGCCGTTC
TCTCCCAATCTCAAAACGCCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT169362 WP_001280945.1 NZ_CP059907:4071052-4071381 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT169362 NZ_CP076412:1712736-1713563 [Ralstonia wenshanensis]
ATGAGCAACGACCTCATTTCAGAAGACGTGCAGGAAAAGTACATCGTGCCGGGCCTCGAACGCGGCCTGCGCCTCCTGTG
CGAATTCAGCCACAAAGACCGCGTCCTGTCGGCGCCGGAACTGGCGCGCCGCCTGAAGGTGCCGCGCTCCACGGTGTTCC
GCCTGCTGACCACACTTGAAGTGATGGGCTTCATCGAGCGCGTAGACGGCGGCCGCGATTTCCGTCTCGGCATGGCGGTG
CTGCGCCTGGGCTTTGAATATCTCGCCTCGCTCGAATTGACCGAGCTGGGCCGCCCGTTGCTCGATCGTCTGCGTGACGA
GATTCACTATCCGTGCAATCTCGTCGTGCGCGATGGCCGGTCGATCGTCTATGTGGCGAAATCGGTCGCGCCCACGCCGT
TCACCAGTTCGGTGAATGTCGGCACGCGCCTGCCGGCTCACGCCACGGTGCTGGGTCGTGTGCTGCTGGCCGATCTGTCG
CTGGCTGAGCTGCGCCAGCTTTATCCGGAGCCGCAGCTGGAAGTGTTTTCCGCCAACACGCCGCGCACGGTGGAAGCGCT
GTTCGAGATGGTCCAGCGCGACCGCGAACACGGCTACGTGCTGCAGGAAGGCTTCTTCGAAGCCAACATCTCGACGATTG
CTGCGCCCGTCCTGGATCGCGCCGGCAAGGTGGTGGCTGCACTCGGCACGACCATTCCGTCGCCGCGCATCGACGCGGCA
CAGCTCGATCTGATGATCGACAAGGTGCGCGCCGCCGCCGGCGAGTTGTCCTACCTGCTGGACTACCGCCCCGCCGGCGG
CAAGGTCGTCAACCTGTTCCGCGAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6