Detailed information of TA system TA169361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3823318..3824102
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5C80_RS18900
• Protein ID: WP_000613624.1
• Coordinates: 3823608..3824102 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: H5C80_RS18895
• Protein ID: WP_001110446.1
• Coordinates: 3823318..3823611 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS18885	3818467..3819426	-	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
H5C80_RS18890	3819999..3823184	+	3186	WP_000827395.1	ribonuclease E	-
H5C80_RS18895	3823318..3823611	+	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
H5C80_RS18900	3823608..3824102	+	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
H5C80_RS18905	3824197..3825150	-	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
H5C80_RS18910	3825162..3826805	-	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
H5C80_RS18915	3826871..3827812	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
H5C80_RS18920	3827812..3828909	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T169361 WP_000613624.1 NZ_CP059907:3823608-3824102 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T169361 NZ_CP076412:957746-958048 [Ralstonia wenshanensis]
ATGCCTAACGACAAACCCGTCAAAACCCGCCCGTGGGACTCCGCCGAGCATCTCAAGACCGAGGCCGATATGGCCGCATA
TCTGGACGCGTGCCTGGAGGAAGCGGCCGACGACCCCGCCTTTATCACCCATGCGCTGGGCGTGGTGGCCCGCGCGCGCG
GCATGACACAACTTGCGCGTGATACCGGCATGACGCGGGAAGGGCTGTACAAGGCCTTGTCCGAAGAAGGCAACCCGAGC
TTTGCCACTGTGCTCAAAGTCATGCGGGCGTTGGGTATTCGCTTGCATGCGGTCGCGGTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT169361 WP_001110446.1 NZ_CP059907:3823318-3823611 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169361 NZ_CP076412:957444-957743 [Ralstonia wenshanensis]
ATGCTGACGATCCGCACCACCAACGCTTTCGACACCTGGTTTTCGAGGCTGCGTGACAAGACTGCGCAGCGCCGTATCCA
GGTGCGCATCGACCGCTTGCAACTGGGTCATCCAGGTGATGTGAAGGCTGTCCGAGCTGGTGTCAGTGAACTACGCATCA
ATCACGGCCCCGGCTACCCGGTGTATTTCGTCCAGCGTGGCGCAGTACTGATCATCCTGTTATGTGGCGGTGACAAGTCG
ACACAGGAAGCCGACATCCGACGCGCGATTGAGTTGTCGCGCCATCTCGACGTGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775