Detailed information of TA system TA169340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1050110..1050705
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: H5C80_RS05080
• Protein ID: WP_000155159.1
• Coordinates: 1050328..1050705 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: H5C80_RS05075
• Protein ID: WP_000557315.1
• Coordinates: 1050110..1050331 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS05050	1045186..1046295	+	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H5C80_RS05055	1046343..1047269	+	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H5C80_RS05060	1047266..1048543	+	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
H5C80_RS05065	1048540..1049307	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H5C80_RS05070	1049309..1050022	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H5C80_RS05075	1050110..1050331	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5C80_RS05080	1050328..1050705	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H5C80_RS05085	1050914..1052230	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H5C80_RS05090	1052406..1053293	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H5C80_RS05095	1053290..1054135	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H5C80_RS05100	1054137..1055207	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1047266..1055947	8681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T169340 WP_000155159.1 NZ_CP059907:1050328-1050705 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T169340 NZ_CP076405:2794852-2795160 [Providencia rettgeri]
ATGCAATATCGCCTCTATCAAAACCGAGATGATGCTATCAAATACCCATATTTACTGGATATTCAAAGTAATATTATTGA
TTTATTGAATACTCGCTTAGTCATCCCGTTATTCGATAGCAGGCTAGTTAAAAAGCCATTACCCGCTCGCTTAAACCCCC
AATTAGTTATCAACGGGCAGGTTTTTATTTTAATGACTCACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTAAGTAGCCAACGAGACACCATTAAACACGCAATTGACTTACTGATTGATGGCTTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT169340 WP_000557315.1 NZ_CP059907:1050110-1050331 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT169340 NZ_CP076405:2794625-2794852 [Providencia rettgeri]
ATGAATAGAACAAAAAAAACCGTCAGTGTCACTGTCGCGCCTGAACTCTACGAACAAGCAAGGCGGGCTAAACTTAACTT
ATCAGCAATACTGGCTTGTGCCTTACAAGCTGAATTAAAACGCCTCGAAGCGCAAAAATGGAAAAATGACAATGCCGAAG
GCTTTAATGAGTTAAACAGGATAAATGAAGAATATGGATTACTCTCAGATAAATACAAGGATTTTTAA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32