Detailed information of TA system TA169339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1022834..1023465
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: H5C80_RS04925
• Protein ID: WP_001260301.1
• Coordinates: 1022834..1023109 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: H5C80_RS04930
• Protein ID: WP_000593557.1
• Coordinates: 1023106..1023465 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS04910	1017838..1018905	+	1068	WP_000361470.1	HlyD family secretion protein	-
H5C80_RS04915	1018902..1021637	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
H5C80_RS04920	1021637..1022761	+	1125	WP_001318088.1	ABC transporter permease	-
H5C80_RS04925	1022834..1023109	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5C80_RS04930	1023106..1023465	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5C80_RS04935	1023585..1023986	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
H5C80_RS04940	1023992..1024798	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
H5C80_RS04945	1024795..1025559	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
H5C80_RS04950	1025559..1026392	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
H5C80_RS04955	1026389..1027333	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T169339 WP_001260301.1 NZ_CP059907:1022834-1023109 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T169339 NZ_CP076405:c1697827-1697642 [Providencia rettgeri]
GTGCAAAGTTCGGAACTGATAAACATACTGCAAAAAAACGGTTGGAAACTTGAAAGAATAAAAGGAAGTCACCATCAGTT
TTCCCACCCACATTTTTCAATTGTAATTACTGTTCCGCATCCACAAAAAGATTTAAAGATAGGCACACTAAATCACATTT
TGAAGGCTGCGAAACTAAAACATTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT169339 WP_000593557.1 NZ_CP059907:1023106-1023465 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT169339 NZ_CP076405:c1697592-1697179 [Providencia rettgeri]
ATGCTTTATACCGCCTTTATTGAAATTGATAATGATGGCAGCGCAAGTGGCTGGTTTCCTGATATTGAAGGATGTACATT
CGCGGGCAGCAATATAGAAGAGGCTTACGCAGAAGCAAAATCAGCAATTGATGCTCATTTTGAATTATTGAGTGAAAAAG
GCTTTGAGATCCCATTATCGAAATCTCAGCAAACGCTATTGAACCCAATACAACCTGAATATGCGCATGGGATATGGTTA
TTTGTGGATGTCGATATGGATAAATACGATGGCCGTACTGAGCGTATTAATATCACCTTGCCGCATCGTTTATTACATCG
TATTGATGCTTTAGTGAAGGTTAATCCTGAATATGGTAGCCGTAGTGGTTTTATTGCTGCTGCAGCTCGTAAAGAATTAA
AAAAAACCGATTAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1