Detailed information of TA system TA169328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 948114..948726
• Replicon: chromosome
• Accession: NZ_CP059903
• Organism: Nocardioides sp. S-713

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H4O22_RS04510
• Protein ID: WP_182527263.1
• Coordinates: 948325..948726 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H4O22_RS04505
• Protein ID: WP_182525866.1
• Coordinates: 948114..948332 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4O22_RS04490	944037..945989	-	1953	WP_182525863.1	Ig-like domain repeat protein	-
H4O22_RS04495	946201..946836	-	636	WP_182525864.1	endonuclease	-
H4O22_RS04500	946916..948085	+	1170	WP_182525865.1	hypothetical protein	-
H4O22_RS04505	948114..948332	+	219	WP_182525866.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H4O22_RS04510	948325..948726	+	402	WP_182527263.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4O22_RS04515	948797..950110	+	1314	WP_182525867.1	hypothetical protein	-
H4O22_RS04520	950180..951442	+	1263	WP_182525868.1	HNH endonuclease	-
H4O22_RS04525	951556..952524	+	969	WP_182525869.1	MsnO8 family LLM class oxidoreductase	-
H4O22_RS04530	952644..953219	+	576	WP_182525870.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-125)

Antitoxin

• Post-segregation antitoxin CcdA (3-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13742.88 Da
• Isoelectric Point: 6.3247

>T169328 WP_182527263.1 NZ_CP059903:948325-948726 [Nocardioides sp. S-713]
VPEGTTSGAVVDASALVDLLAGTELAGAVRARLAGLVLHAPAHLDVECLSALGRLHRAELLTSAQVSTALSDLAEAPLLR
HPLPPLLAGAWSRRENLRLADSLYVELAEHLGLPVVTTDARLARGAARVALIG

Nucleotide

• Length: 402 bp

>T169328 NZ_CP076404:c2857782-2857447 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7359.22 Da
• Isoelectric Point: 4.5203

>AT169328 WP_182525866.1 NZ_CP059903:948114-948332 [Nocardioides sp. S-713]
MARLNVYVPDELAAAARDAGLNVSALTQQALSDALSRAATDSWLARIDRPVSAATHAAVAAALDAARDELGA

Nucleotide

• Length: 219 bp

>AT169328 NZ_CP076404:c2858030-2857782 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA