Detailed information of TA system TA169325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 168801..169339
• Replicon: chromosome
• Accession: NZ_CP059901
• Organism: Mumia sp. ZJ1417

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H4N58_RS00840
• Protein ID: WP_167000994.1
• Coordinates: 169037..169339 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: H4N58_RS00835
• Protein ID: WP_167249463.1
• Coordinates: 168801..169037 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4N58_RS00810	164353..166458	+	2106	WP_167249469.1	iron ABC transporter permease	-
H4N58_RS00815	166529..166807	-	279	WP_167000986.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
H4N58_RS00820	166911..167573	+	663	WP_167000987.1	response regulator transcription factor	-
H4N58_RS00825	167579..167917	-	339	WP_167249467.1	cytoplasmic protein	-
H4N58_RS00830	167961..168638	-	678	WP_167249465.1	Type 1 glutamine amidotransferase-like domain-containing protein	-
H4N58_RS00835	168801..169037	+	237	WP_167249463.1	ribbon-helix-helix domain-containing protein	Antitoxin
H4N58_RS00840	169037..169339	+	303	WP_167000994.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H4N58_RS00845	169683..169916	+	234	WP_167000996.1	ribbon-helix-helix protein, CopG family	-
H4N58_RS00850	169913..170155	+	243	WP_167249461.1	toxin	-
H4N58_RS00855	170181..170447	-	267	WP_167249460.1	hypothetical protein	-
H4N58_RS00860	170605..170904	-	300	WP_167249458.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10896.62 Da
• Isoelectric Point: 6.4568

>T169325 WP_167000994.1 NZ_CP059901:169037-169339 [Mumia sp. ZJ1417]
MRPIHVARLDKARPVLVLTREVVRPYLNRVTVAPITSTIRGLSTEVPVGPINGLDRDSVVSCDNIVTVPVESVGRQLGFL
LESQEGDLAHAIRAAFDLDL

Nucleotide

• Length: 303 bp

>T169325 NZ_CP076404:c2048209-2047958 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8475.35 Da
• Isoelectric Point: 4.0048

>AT169325 WP_167249463.1 NZ_CP059901:168801-169037 [Mumia sp. ZJ1417]
MSTQIAVRLDDDLVTFVDELVKAGEAPSRAAVVTQALQREQRRTVAERDAAILAAEREDTDMDGLAEFAAGVSMDDHD

Nucleotide

• Length: 237 bp

>AT169325 NZ_CP076404:c2047961-2047707 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA