Detailed information of TA system TA169323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4165932..4166582
• Replicon: chromosome
• Accession: NZ_CP059900
• Organism: Tomitella sp. HY172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H4F70_RS19265
• Protein ID: WP_182360543.1
• Coordinates: 4165932..4166354 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H4F70_RS19270
• Protein ID: WP_182358398.1
• Coordinates: 4166367..4166582 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F70_RS19255	4162511..4164655	-	2145	WP_182358396.1	heavy metal translocating P-type ATPase	-
H4F70_RS19260	4164971..4165849	+	879	WP_182358397.1	DUF559 domain-containing protein	-
H4F70_RS19265	4165932..4166354	-	423	WP_182360543.1	PIN domain-containing protein	Toxin
H4F70_RS19270	4166367..4166582	-	216	WP_182358398.1	ribbon-helix-helix domain-containing protein	Antitoxin
H4F70_RS19275	4166685..4168772	-	2088	WP_182360544.1	enoyl-CoA hydratase/isomerase family protein	-
H4F70_RS19280	4168820..4169818	-	999	WP_182360545.1	NADPH:quinone oxidoreductase family protein	-
H4F70_RS19285	4169901..4170926	-	1026	WP_182360546.1	phosphotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-118)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-37)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14676.41 Da
• Isoelectric Point: 4.1508

>T169323 WP_182360543.1 NZ_CP059900:c4166354-4165932 [Tomitella sp. HY172]
MIVDTSALLAFFDASEPDHAAVSAALTAEAGALVVSPFVVAELDYLVAARIGVDAELAVLDELAQGAWDLPEFGAAELAE
ARAVVQRYRDQDIGVADASNVVLAGRYGTDRIATLDHRHFSVLHTDRGGAFTLVPGDAGR

Nucleotide

• Length: 423 bp

>T169323 NZ_CP076404:2036065-2036439 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7633.68 Da
• Isoelectric Point: 11.3450

>AT169323 WP_182358398.1 NZ_CP059900:c4166582-4166367 [Tomitella sp. HY172]
MKKTTLYLPDELKAAVARSAAREGVSEAEVIRRSIARSVGGERPRPRGGLFRSGSPIAREADAHLAGFGER

Nucleotide

• Length: 216 bp

>AT169323 NZ_CP076404:2035608-2035976 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA