Detailed information of TA system TA169322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4153736..4154340
• Replicon: chromosome
• Accession: NZ_CP059900
• Organism: Tomitella sp. HY172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H4F70_RS19210
• Protein ID: WP_182358389.1
• Coordinates: 4153736..4154077 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: H4F70_RS19215
• Protein ID: WP_182348485.1
• Coordinates: 4154071..4154340 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F70_RS19190	4151239..4151580	-	342	WP_182358386.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H4F70_RS19195	4151577..4151780	-	204	WP_182358387.1	DUF3018 family protein	-
H4F70_RS19200	4151968..4152966	+	999	WP_182360541.1	agmatinase	-
H4F70_RS19205	4152981..4153739	-	759	WP_182358388.1	alpha/beta hydrolase	-
H4F70_RS19210	4153736..4154077	-	342	WP_182358389.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H4F70_RS19215	4154071..4154340	-	270	WP_182348485.1	ribbon-helix-helix protein, CopG family	Antitoxin
H4F70_RS19220	4154497..4156644	+	2148	WP_182358390.1	FAD-dependent oxidoreductase	-
H4F70_RS19225	4156676..4156999	+	324	Protein_3779	transposase	-
H4F70_RS19230	4157891..4158388	-	498	WP_182358391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 11739.55 Da
• Isoelectric Point: 8.6160

>T169322 WP_182358389.1 NZ_CP059900:c4154077-4153736 [Tomitella sp. HY172]
VVIRRGALYWADVGPAVGSRPAERRPVLVIQANPYNDSRLATAVAAVVTSNTALAAMPGNVFLPAAVTGLPRDSVVNVTA
LVTLDKGDLTEQAGVVPAAILDEVDRGLRRLGL

Nucleotide

• Length: 342 bp

>T169322 NZ_CP076404:c1881143-1881040 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9304.16 Da
• Isoelectric Point: 4.0886

>AT169322 WP_182348485.1 NZ_CP059900:c4154340-4154071 [Tomitella sp. HY172]
MKTAISLPDDTYDRATRRAGELGLSRSEFFARAALRYLDELDARSLTARIDEALDTVAAAGAGDAEGDSGSGDAIAAGRA
VLGALDDEW

Nucleotide

• Length: 270 bp

>AT169322 NZ_CP076404:1881002-1881273 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT