Detailed information of TA system TA169309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 139014..139759
• Replicon: plasmid pKP18069-CTX
• Accession: NZ_CP059891
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KP18069

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: H4N62_RS27165
• Protein ID: WP_032408901.1
• Coordinates: 139014..139505 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: H4N62_RS27170
• Protein ID: WP_014386183.1
• Coordinates: 139493..139759 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4N62_RS27140	134698..135453	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
H4N62_RS27145	135715..136155	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
H4N62_RS27150	136152..136502	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
H4N62_RS27155	136533..138125	+	1593	Protein_147	IS66 family transposase	-
H4N62_RS27160	138322..138573	+	252	WP_032408900.1	aldo/keto reductase	-
H4N62_RS27165	139014..139505	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
H4N62_RS27170	139493..139759	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
H4N62_RS27175	139956..140114	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
H4N62_RS27180	140358..140843	-	486	WP_014386185.1	hypothetical protein	-
H4N62_RS27185	141033..141305	+	273	WP_032408902.1	hypothetical protein	-
H4N62_RS27190	141302..141652	+	351	WP_014386186.1	hypothetical protein	-
H4N62_RS27195	142289..142615	+	327	WP_014386187.1	hypothetical protein	-
H4N62_RS27200	142677..142889	+	213	WP_014386188.1	hypothetical protein	-
H4N62_RS27205	142900..143124	+	225	WP_014386189.1	hypothetical protein	-
H4N62_RS27210	143205..143525	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H4N62_RS27215	143515..143793	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
H4N62_RS27220	143794..144207	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / mph(A) / blaCTX-M-15	-	1..189307	189307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T169309 WP_032408901.1 NZ_CP059891:c139505-139014 [Klebsiella pneumoniae subsp. pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T169309 NZ_CP076386:c3530728-3530525 [Dickeya dadantii]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACTATCGACACGCTTGAACGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGACGAGCTTGAAATGTTCTATTCAGCTGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAAATACGTTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT169309 WP_014386183.1 NZ_CP059891:c139759-139493 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT169309 NZ_CP076386:c3531150-3530782 [Dickeya dadantii]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCGTGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCAGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGTTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATAAATGACGCCGAGTTGCGGAAATGGCAAAAATCCAGGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6