Detailed information of TA system TA169308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 115220..115863
• Replicon: plasmid pKP18069-CTX
• Accession: NZ_CP059891
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KP18069

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H4N62_RS27035
• Protein ID: WP_014386165.1
• Coordinates: 115220..115636 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: H4N62_RS27040
• Protein ID: WP_032408893.1
• Coordinates: 115633..115863 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4N62_RS27010	111840..112271	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
H4N62_RS27015	112268..112996	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
H4N62_RS27020	112993..113319	+	327	WP_001568059.1	hypothetical protein	-
H4N62_RS27025	113375..113749	+	375	WP_032408891.1	hypothetical protein	-
H4N62_RS27030	113929..114897	-	969	WP_074422984.1	IS5 family transposase	-
H4N62_RS27035	115220..115636	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
H4N62_RS27040	115633..115863	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H4N62_RS27045	116183..116617	+	435	WP_000103648.1	RidA family protein	-
H4N62_RS27050	116622..117296	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
H4N62_RS27055	117312..118469	+	1158	WP_032408895.1	acetylornithine deacetylase	-
H4N62_RS27060	118640..119788	+	1149	WP_094965598.1	ABC transporter ATP-binding protein	-
H4N62_RS27065	119795..120790	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / mph(A) / blaCTX-M-15	-	1..189307	189307
	-	flank	IS/Tn	-	-	113929..114897	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T169308 WP_014386165.1 NZ_CP059891:c115636-115220 [Klebsiella pneumoniae subsp. pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T169308 NZ_CP076386:3059639-3060070 [Dickeya dadantii]
GTGGCCCAGTGGCAATGTGATTTACGCGTATCCTGGCGCATGCAGTTGTTTTCGCTGCTGACGCACGGGTTTCTGGTGCT
GATGATCCTGCTGGCTCCCTGGCCGGACGGCTATGCGCCGCTGTGGCTGGGTCTGGTGACGCTGGTAGTGTTCGGTTTTG
TGTGCAGCCAGCGCATCATCAAGACGCGTCAGGGGGAGATTTCCCTGCATGGGGAAAACCAACTGCACTGGCAGCAGCGT
GACTGGCAGATAGTCCGGCGGCCATGGCTGATGCGCAACGGCGTGTTGCTGTCATTGCGGGCGGTGGATGGCAAAGGGCA
TCAGCGCTTATGGCTGGCGTCAGACAGCATGGGCAACGAAGAGTGGCGGTTGTTGCGTCAACTGTTGCAGCAGCATTCGC
TGCAGGGAACGGAGTCCTCCCACCATCATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT169308 WP_032408893.1 NZ_CP059891:c115863-115633 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT169308 NZ_CP076386:3059392-3059658 [Dickeya dadantii]
ATGGACATCAATAACAAATCACGTATTCACTGGGCGTGCAGACGCGGAATGCGTGAACTGGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGTTGAGCGACGACGACAAGCAGCAGTTCATCCGTCTGCTGCAGTGCGACGATCCCGACT
TGTTCAACTGGTTGATGAACCATGGCGAACCGGTGGATCCGGCGCTGAAACGTATGGTTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA