Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 74834..75477 Replicon plasmid pKP18069-KPC
Accession NZ_CP059890
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP18069

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H4N62_RS26290 Protein ID WP_001044770.1
Coordinates 75061..75477 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H4N62_RS26285 Protein ID WP_001261282.1
Coordinates 74834..75064 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4N62_RS26245 70130..70309 + 180 WP_006788217.1 hypothetical protein -
H4N62_RS26250 70797..71591 - 795 WP_004197635.1 site-specific integrase -
H4N62_RS26255 71789..72805 - 1017 WP_017899884.1 hypothetical protein -
H4N62_RS26260 72816..73130 - 315 WP_053389906.1 hypothetical protein -
H4N62_RS26265 73157..73552 - 396 WP_017899885.1 hypothetical protein -
H4N62_RS26270 73721..74026 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
H4N62_RS26275 74028..74246 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
H4N62_RS26280 74416..74877 - 462 WP_160880186.1 hypothetical protein -
H4N62_RS26285 74834..75064 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H4N62_RS26290 75061..75477 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H4N62_RS26295 75551..77113 + 1563 WP_004206609.1 AAA family ATPase -
H4N62_RS26300 77098..78120 + 1023 WP_000361404.1 helicase UvrD -
H4N62_RS26305 78376..79073 + 698 Protein_91 IS1 family transposase -
H4N62_RS26310 79102..79374 + 273 Protein_92 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..95050 95050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T169306 WP_001044770.1 NZ_CP059890:75061-75477 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T169306 NZ_CP076386:2784887-2785207 [Dickeya dadantii]
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATAACCCCTGTGCGTCACCTGTAGCCGTCTGGCAGCAACTACTGAC
TTACCTGCTGGAAAAGCACTACGGCCTGACGCTGAACGACACACCATTCTGTGAGGAAAACGTCATTCAGGCGCATATCG
ATGCTGGCGTCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTCACTGCCGTTGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAGGCATA
A

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT169306 WP_001261282.1 NZ_CP059890:74834-75064 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT169306 NZ_CP076386:2784482-2784817 [Dickeya dadantii]
ATGCCATCATCGGATACCCCGGAGTGGGGATTAAAATGTGCCATCACTCGGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTAATTAAGCTGTTGGAACAGAAGCTACGCATGGGGGAACTGAATCCCCGCCAGCAGAGTTGTGTCACG
TTGTATTGTAACGAATGGACTTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
AGCGGCAACACAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References