Detailed information of TA system TA169305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 73721..74246
• Replicon: plasmid pKP18069-KPC
• Accession: NZ_CP059890
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KP18069

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: H4N62_RS26270
• Protein ID: WP_013023785.1
• Coordinates: 73721..74026 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: H4N62_RS26275
• Protein ID: WP_001568025.1
• Coordinates: 74028..74246 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4N62_RS26240	69384..70010	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
H4N62_RS26245	70130..70309	+	180	WP_006788217.1	hypothetical protein	-
H4N62_RS26250	70797..71591	-	795	WP_004197635.1	site-specific integrase	-
H4N62_RS26255	71789..72805	-	1017	WP_017899884.1	hypothetical protein	-
H4N62_RS26260	72816..73130	-	315	WP_053389906.1	hypothetical protein	-
H4N62_RS26265	73157..73552	-	396	WP_017899885.1	hypothetical protein	-
H4N62_RS26270	73721..74026	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
H4N62_RS26275	74028..74246	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H4N62_RS26280	74416..74877	-	462	WP_160880186.1	hypothetical protein	-
H4N62_RS26285	74834..75064	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H4N62_RS26290	75061..75477	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
H4N62_RS26295	75551..77113	+	1563	WP_004206609.1	AAA family ATPase	-
H4N62_RS26300	77098..78120	+	1023	WP_000361404.1	helicase UvrD	-
H4N62_RS26305	78376..79073	+	698	Protein_91	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..95050	95050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T169305 WP_013023785.1 NZ_CP059890:c74026-73721 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T169305 NZ_CP076386:c998648-998244 [Dickeya dadantii]
ATGAGTATCAGGATTTTCAAGTCAGCACTGATCCGTCAGCAATTAAGCCAACAAGAGCTTGATGATTTGGCAGCGGATTT
TCTGTCCTACAAAAAAGACGGTGTATTGCCAGATATCTTTGGGCGTGATGCACCTTACGATGATGACCGCACCTATCCCT
TGGTAAAAGAAGAGCAGGTGGCCCACATTCATCTTGCCGATGCGGATGCCCCTTTCCCGAAGTTTTTACGTCAGTTCAAA
CGAACCAGCGATCAGGCGCATTTGGTATACTGCCAAGGTGCGATGGAGCCCAATGCCTATCTGTTGATTATCATCCTTAA
ACCCGAAGCACATAAAATGGCGCGCAACAATAACCATATGCATAAGATCGGGATGATGGCAGAAGCGTTCAGGATGAAGC
ATTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT169305 WP_001568025.1 NZ_CP059890:c74246-74028 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT169305 NZ_CP076386:c998938-998645 [Dickeya dadantii]
ATGGAACGCATCCTCGCAGAAAAATCTATCAACATCACCGAGTTAAGAAAGAACCCGGCGAAATATTTTATTGATGAGCC
GGTTGCCGTTCTCTCCAACAACCGTCCCGCTGGCTACATGGTCAGTGCTAAGGTGTTTGAAGAGCTGATCGATCTGTTGG
AAGAGAAACAAGGCAGGGTTCATACCGCTGCGCGATTCAGGCCATCTGCTGAACGTTTAAGCGATATTGCGGATAGCGGG
CAGAAACTGCTGCAAAACGCCTCTGACAAGGATCTGACCGAGTTCACGGAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8