Detailed information of TA system TA169284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 323642..324559
• Replicon: chromosome
• Accession: NZ_CP059855
• Organism: Bacillus velezensis strain Pm9

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: H4K32_RS01405
• Protein ID: WP_003154806.1
• Coordinates: 323642..324388 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: H4K32_RS01410
• Protein ID: WP_003154807.1
• Coordinates: 324389..324559 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4K32_RS01385	319033..319983	-	951	WP_024085196.1	ring-cleaving dioxygenase	-
H4K32_RS01390	320307..321623	+	1317	WP_003154801.1	amino acid permease	-
H4K32_RS01395	321909..322526	+	618	WP_003154804.1	DUF47 domain-containing protein	-
H4K32_RS01400	322539..323537	+	999	WP_003154805.1	inorganic phosphate transporter	-
H4K32_RS01405	323642..324388	+	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H4K32_RS01410	324389..324559	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H4K32_RS01415	324816..325694	-	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
H4K32_RS01420	325708..325971	-	264	WP_003154813.1	phage holin	-
H4K32_RS01425	325985..326248	-	264	WP_032866112.1	hemolysin XhlA family protein	-
H4K32_RS01430	326300..327061	-	762	WP_182274940.1	phage portal protein	-
H4K32_RS01435	327118..327315	-	198	WP_003154819.1	XkdX family protein	-
H4K32_RS01440	327321..327692	-	372	WP_014304856.1	hypothetical protein	-
H4K32_RS01445	327705..329327	-	1623	WP_003154821.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	321909..361269	39360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T169284 WP_003154806.1 NZ_CP059855:323642-324388 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T169284 NZ_CP076365:58273-58683 [Gemmobacter fulva]
ATGAGCGCTGAGTTCGCGGATACGAATGTCGTTCTTTACCTGCTCGACGACGGCTCGAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAAGTCCTGAACGAGACGCTGGTCAACTGTCGCCGCAAGGCTGGCCTGAGTT
GGGAGGAAGCAGGGGCGTTTCTCGAGGGCGTGCGCGCCTTGTGTCCCGTCGAAGACCTGACCCTTCAGACCCATGACGTC
GGCCGCGCCTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCAGGCTGCAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAACTTCGCATCATCAACCCCTTTGCGCGAGCGG
ACAGTTTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT169284 WP_003154807.1 NZ_CP059855:324389-324559 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT169284 NZ_CP076365:58056-58283 [Gemmobacter fulva]
ATGCAGGTCGCAAAATGGGGCAACTCGCTTGCCGTCCGCCTGCCCGCCGAGCTTGTTCGGGAGCTTGGCCTCAAGGAAGG
CGATCAGATCGACCTGGTCAAGGACGACCGCCAGGTCAGAGTCCGTCGTCTGGCGCGCGCGGATGAGGTGCTGACGGGTC
TGCGCCAGTTCCGGGGCAAGCTGCCCGCTGCAGAGCGCCTGAGCCGCGATGACGCGCATGAGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14