Detailed information of TA system TA169270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 349860..351288
• Replicon: chromosome
• Accession: NZ_CP059851
• Organism: Sandaracinobacter sp. M6

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: H3309_RS01760
• Protein ID: WP_182296958.1
• Coordinates: 350413..351288 (+)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: H3309_RS01755
• Protein ID: WP_207791543.1
• Coordinates: 349860..350420 (+)
• Length: 187 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3309_RS01740	345230..345904	+	675	WP_182296950.1	hypothetical protein	-
H3309_RS01745	345908..347620	+	1713	WP_182296952.1	ATP-binding protein	-
H3309_RS01750	347691..349718	+	2028	WP_182296954.1	DUF4338 domain-containing protein	-
H3309_RS01755	349860..350420	+	561	WP_207791543.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
H3309_RS01760	350413..351288	+	876	WP_182296958.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H3309_RS01765	351764..351970	+	207	WP_182296960.1	hypothetical protein	-
H3309_RS01770	352041..353138	+	1098	WP_182296962.1	ester cyclase	-
H3309_RS01775	353197..354360	+	1164	WP_182296964.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tufA	253265..468649	215384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-262)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (2-72)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32319.96 Da
• Isoelectric Point: 4.6785

>T169270 WP_182296958.1 NZ_CP059851:350413-351288 [Sandaracinobacter sp. M6]
MAKTPTDMAKSVKDRLLNIARQEGRVFDVLLVRFALERLLYRLSISEHRDRFVLKGGMLVTVWVEDDNRVTRDADFLGHG
DPNPERLTADFREIMAIESEDGLVFDLDSLAATAIREEMEYGGTRLKTAAYLEKTRIPVTIDIGFGDAMADATQRLDYPT
LLDLPAPNVRAYTPATVIAEKFQAMVALGVLNGRMKDYYDLWAIPRALNIAAEDLDAAITATFERRQTAIPTERPPGLSA
EMVDDEVKQRQWRAYAASVELEGVSLESIVDTVWGLVGPSCARIAAKAAKA

Nucleotide

• Length: 876 bp

>T169270 NZ_CP076344:c2593248-2593146 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 187 a.a.
• Molecular weight: 20557.75 Da
• Isoelectric Point: 9.9816

>AT169270 WP_207791543.1 NZ_CP059851:349860-350420 [Sandaracinobacter sp. M6]
MMRAHELREAGISAQTIARAVETGDIERISRGVYQRRGSEIEENQILAEATMRVPKGVIALVSALAFHGLTDQMPRRIWM
AIGASDWAPVQAYPPMRMVRFTEPYLRQGVENHMIAGLQVPIYSVPKTLADLFRNSKLVDRSVAVEGLRATIEQRKATPS
AIAQGAKDGGAWKIMQPYLEALTSNG

Nucleotide

• Length: 561 bp

>AT169270 NZ_CP076344:2593108-2593376 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT