Detailed information of TA system TA169269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 309841..311348
• Replicon: chromosome
• Accession: NZ_CP059851
• Organism: Sandaracinobacter sp. M6

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: H3309_RS01555
• Protein ID: WP_182296880.1
• Coordinates: 310080..311348 (+)
• Length: 423 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: H3309_RS01550
• Protein ID: WP_182296878.1
• Coordinates: 309841..310083 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3309_RS01530	305491..307929	-	2439	WP_182296874.1	conjugal transfer protein TrbE	-
H3309_RS01535	307939..308220	-	282	WP_062765738.1	VirB3 family type IV secretion system protein	-
H3309_RS01540	308220..308552	-	333	WP_035599872.1	TrbC/VirB2 family protein	-
H3309_RS01545	308549..309538	-	990	WP_182296876.1	P-type conjugative transfer ATPase TrbB	-
H3309_RS01550	309841..310083	+	243	WP_182296878.1	helix-turn-helix transcriptional regulator	Antitoxin
H3309_RS01555	310080..311348	+	1269	WP_182296880.1	type II toxin-antitoxin system HipA family toxin	Toxin
H3309_RS01560	311504..312304	+	801	WP_182296882.1	alpha/beta hydrolase	-
H3309_RS01565	312348..312794	-	447	WP_182296884.1	YdeI/OmpD-associated family protein	-
H3309_RS01570	312851..313069	-	219	WP_182296886.1	DUF4287 domain-containing protein	-
H3309_RS01575	313130..313498	-	369	WP_182296888.1	DoxX family protein	-
H3309_RS01580	313588..314205	-	618	WP_182296890.1	TetR/AcrR family transcriptional regulator	-
H3309_RS01585	314443..314865	-	423	WP_182296892.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tufA	253265..468649	215384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 423 a.a.
• Molecular weight: 45448.16 Da
• Isoelectric Point: 5.5670

>T169269 WP_182296880.1 NZ_CP059851:310080-311348 [Sandaracinobacter sp. M6]
MTRTLDIWWDGRLVGQLTQDKHGELGFAYARAWLDDEEAQPLSASLPKRAEPFTRRECRPYFSGLLPEESQRDAAAQALG
VSRANDFALLDRLGGDVAGALQLLPPGEVPATLAPDQRPTPLDDAGLIRVLDALPVRPLLAGEEGLRLSLAGAQSKVPVV
LVDGVVALPAPGQPTTHILKPPISRFAATTENEAFVMRLAAAIGFDVAPVEVRIVQDRTFLLVQRYDRATGDDGFVRRIH
QEDFCQALGVPPETKYASEGGPTFKDCFALLRRVVARPAVDVLKLLDAVIFNVIAGNADAHGKNFSILYGAEGPRLAPLY
DLLATVAYPDLSPKFAMKIGRRATLAELDADGWAAFAADAGVGLPLVRRRIAEISKAVLEKATAVGAELSRPGLDRTAIA
RFAEMIRERGERCALTVAGPSQ

Nucleotide

• Length: 1269 bp

>T169269 NZ_CP076344:c2273571-2273293 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8273.57 Da
• Isoelectric Point: 10.5957

>AT169269 WP_182296878.1 NZ_CP059851:309841-310083 [Sandaracinobacter sp. M6]
MARRNLTTAEIGDIVRTTRKAAGLRQDELAGAAGVGLRFIVDLEAGKATAQIGKTLQVLSALGCSLDITPPSDPKGARKA

Nucleotide

• Length: 243 bp

>AT169269 NZ_CP076344:c2273293-2273009 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA