Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4765545..4766161 Replicon chromosome
Accession NZ_CP059849
Organism Citrobacter freundii strain 3863

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag H4O05_RS22865 Protein ID WP_003028682.1
Coordinates 4765545..4765919 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag H4O05_RS22870 Protein ID WP_043018956.1
Coordinates 4765919..4766161 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4O05_RS22850 (4763048) 4763048..4763950 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
H4O05_RS22855 (4763947) 4763947..4764582 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
H4O05_RS22860 (4764579) 4764579..4765508 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
H4O05_RS22865 (4765545) 4765545..4765919 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
H4O05_RS22870 (4765919) 4765919..4766161 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
H4O05_RS22875 (4766367) 4766367..4767275 + 909 WP_096890303.1 alpha/beta hydrolase -
H4O05_RS22880 (4767426) 4767426..4768367 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
H4O05_RS22885 (4768412) 4768412..4768849 - 438 WP_003840439.1 D-aminoacyl-tRNA deacylase -
H4O05_RS22890 (4768846) 4768846..4769718 - 873 WP_003840437.1 virulence factor BrkB family protein -
H4O05_RS22895 (4769712) 4769712..4770311 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T169267 WP_003028682.1 NZ_CP059849:c4765919-4765545 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T169267 NZ_CP076344:1230653-1230955 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT169267 WP_043018956.1 NZ_CP059849:c4766161-4765919 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT169267 NZ_CP076344:1230991-1231332 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References