Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3679860..3680480 Replicon chromosome
Accession NZ_CP059849
Organism Citrobacter freundii strain 3863

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4O05_RS17895 Protein ID WP_002892050.1
Coordinates 3680262..3680480 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag H4O05_RS17890 Protein ID WP_003021733.1
Coordinates 3679860..3680234 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4O05_RS17880 (3675006) 3675006..3676199 + 1194 WP_088902653.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4O05_RS17885 (3676222) 3676222..3679371 + 3150 WP_003021736.1 efflux RND transporter permease AcrB -
H4O05_RS17890 (3679860) 3679860..3680234 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
H4O05_RS17895 (3680262) 3680262..3680480 + 219 WP_002892050.1 HHA domain-containing protein Toxin
H4O05_RS17900 (3680787) 3680787..3681338 + 552 WP_185229111.1 maltose O-acetyltransferase -
H4O05_RS17905 (3681455) 3681455..3681925 + 471 WP_119173998.1 YlaC family protein -
H4O05_RS17910 (3682004) 3682004..3682144 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
H4O05_RS17915 (3682146) 3682146..3682406 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
H4O05_RS17920 (3682595) 3682595..3684148 + 1554 WP_003835927.1 EAL domain-containing protein -
H4O05_RS17925 (3684200) 3684200..3684553 - 354 WP_003831033.1 DUF1428 family protein -
H4O05_RS17930 (3684618) 3684618..3685247 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169266 WP_002892050.1 NZ_CP059849:3680262-3680480 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T169266 NZ_CP076344:c1202868-1202650 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT169266 WP_003021733.1 NZ_CP059849:3679860-3680234 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT169266 NZ_CP076344:c1203268-1202894 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References