Detailed information of TA system TA169264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2404511..2405150
• Replicon: chromosome
• Accession: NZ_CP059849
• Organism: Citrobacter freundii strain 3863

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: H4O05_RS11720
• Protein ID: WP_003020221.1
• Coordinates: 2404511..2404687 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H4O05_RS11725
• Protein ID: WP_123924900.1
• Coordinates: 2404734..2405150 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4O05_RS11700 (2401292)	2401292..2401522	-	231	WP_003020233.1	DUF2554 family protein	-
H4O05_RS11705 (2401712)	2401712..2401837	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
H4O05_RS11710 (2401837)	2401837..2402847	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
H4O05_RS11715 (2402847)	2402847..2404250	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
H4O05_RS11720 (2404511)	2404511..2404687	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
H4O05_RS11725 (2404734)	2404734..2405150	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H4O05_RS11730 (2405243)	2405243..2406652	+	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
H4O05_RS11735 (2406981)	2406981..2408126	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
H4O05_RS11740 (2408143)	2408143..2409159	+	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
H4O05_RS11745 (2409160)	2409160..2410104	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T169264 WP_003020221.1 NZ_CP059849:2404511-2404687 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T169264 NZ_CP076344:728247-728561 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT169264 WP_123924900.1 NZ_CP059849:2404734-2405150 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT169264 NZ_CP076344:728011-728244 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A8B5QF36