Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 809405..810059 Replicon chromosome
Accession NZ_CP059849
Organism Citrobacter freundii strain 3863

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag H4O05_RS03925 Protein ID WP_003026936.1
Coordinates 809652..810059 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag H4O05_RS03920 Protein ID WP_003026938.1
Coordinates 809405..809671 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4O05_RS03895 (804606) 804606..806039 - 1434 WP_048218552.1 6-phospho-beta-glucosidase BglA -
H4O05_RS03900 (806160) 806160..806888 - 729 WP_060855085.1 MurR/RpiR family transcriptional regulator -
H4O05_RS03905 (806941) 806941..807252 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
H4O05_RS03910 (807415) 807415..808074 + 660 WP_003026947.1 hemolysin III family protein -
H4O05_RS03915 (808168) 808168..809148 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
H4O05_RS03920 (809405) 809405..809671 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
H4O05_RS03925 (809652) 809652..810059 + 408 WP_003026936.1 protein YgfX Toxin
H4O05_RS03930 (810104) 810104..810625 - 522 WP_003026933.1 flavodoxin FldB -
H4O05_RS03935 (810739) 810739..811635 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
H4O05_RS03940 (811659) 811659..812372 + 714 WP_185228988.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H4O05_RS03945 (812378) 812378..814111 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T169254 WP_003026936.1 NZ_CP059849:809652-810059 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T169254 NZ_CP076318:3863407-3863757 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT169254 WP_003026938.1 NZ_CP059849:809405-809671 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT169254 NZ_CP076318:3863162-3863413 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References