Detailed information of TA system TA169250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 77219..78022
• Replicon: plasmid pMAR2
• Accession: NZ_CP059841
• Organism: Escherichia coli strain E2348/69

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: Q0H047
• Locus tag: H4J01_RS25005
• Protein ID: WP_000348885.1
• Coordinates: 77219..77749 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: H4J01_RS25010
• Protein ID: WP_001275013.1
• Coordinates: 77753..78022 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J01_RS24975	72343..72648	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-
H4J01_RS24980	72650..72868	-	219	WP_000813637.1	type II toxin-antitoxin system antitoxin CcdA	-
H4J01_RS24985	73582..76203	+	2622	WP_000283686.1	hypothetical protein	-
H4J01_RS24990	76402..76632	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H4J01_RS24995	76808..76996	+	189	WP_001386137.1	hypothetical protein	-
H4J01_RS25000	77001..77218	-	218	Protein_96	transposase	-
H4J01_RS25005	77219..77749	-	531	WP_000348885.1	GNAT family N-acetyltransferase	Toxin
H4J01_RS25010	77753..78022	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
H4J01_RS25015	78910..79899	+	990	WP_024201093.1	RepB family plasmid replication initiator protein	-
H4J01_RS25020	80222..80872	-	651	Protein_100	tyrosine-type recombinase/integrase	-
H4J01_RS25025	81293..81874	+	582	WP_000253757.1	bundle-forming pilus major subunit BfpA	-
H4J01_RS25030	81921..82322	+	402	WP_001261182.1	bundle-forming pilus protein BfpG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	toxB / toxB / bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL	1..96603	96603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (93-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20319.23 Da
• Isoelectric Point: 6.4889

>T169250 WP_000348885.1 NZ_CP059841:c77749-77219 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKVLRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVILGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T169250 NZ_CP076318:c2584560-2584246 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT169250 WP_001275013.1 NZ_CP059841:c78022-77753 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT169250 NZ_CP076318:c2584249-2583833 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: Q0H047

Antitoxin

• AlphaFold DB: G3CAI7