Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72343..72868 Replicon plasmid pMAR2
Accession NZ_CP059841
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H4J01_RS24975 Protein ID WP_001159871.1
Coordinates 72343..72648 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q0H051
Locus tag H4J01_RS24980 Protein ID WP_000813637.1
Coordinates 72650..72868 (-) Length 73 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS24945 67892..68880 + 989 Protein_85 translesion error-prone DNA polymerase V subunit UmuC -
H4J01_RS24950 68885..69277 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
H4J01_RS24955 69282..70253 - 972 WP_001103698.1 hypothetical protein -
H4J01_RS24960 70482..71126 + 645 WP_000633911.1 AAA family ATPase -
H4J01_RS24965 71120..71395 + 276 WP_000239529.1 hypothetical protein -
H4J01_RS24970 71533..72342 - 810 WP_000016979.1 site-specific integrase -
H4J01_RS24975 72343..72648 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H4J01_RS24980 72650..72868 - 219 WP_000813637.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H4J01_RS24985 73582..76203 + 2622 WP_000283686.1 hypothetical protein -
H4J01_RS24990 76402..76632 + 231 WP_001261274.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H4J01_RS24995 76808..76996 + 189 WP_001386137.1 hypothetical protein -
H4J01_RS25000 77001..77218 - 218 Protein_96 transposase -
H4J01_RS25005 77219..77749 - 531 WP_000348885.1 GNAT family N-acetyltransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - toxB / toxB / bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL 1..96603 96603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-100)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T169249 WP_001159871.1 NZ_CP059841:c72648-72343 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T169249 NZ_CP076318:c2518919-2518623 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8428.45 Da        Isoelectric Point: 4.7405

>AT169249 WP_000813637.1 NZ_CP059841:c72868-72650 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKVENQEGMVEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT169249 NZ_CP076318:c2518621-2518226 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CCE8


Antitoxin

Source ID Structure
AlphaFold DB Q0H051

References