Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 72343..72868 | Replicon | plasmid pMAR2 |
Accession | NZ_CP059841 | ||
Organism | Escherichia coli strain E2348/69 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | S1PFV8 |
Locus tag | H4J01_RS24975 | Protein ID | WP_001159871.1 |
Coordinates | 72343..72648 (-) | Length | 102 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | Q0H051 |
Locus tag | H4J01_RS24980 | Protein ID | WP_000813637.1 |
Coordinates | 72650..72868 (-) | Length | 73 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H4J01_RS24945 | 67892..68880 | + | 989 | Protein_85 | translesion error-prone DNA polymerase V subunit UmuC | - |
H4J01_RS24950 | 68885..69277 | - | 393 | WP_000340833.1 | plasmid partitioning/stability family protein | - |
H4J01_RS24955 | 69282..70253 | - | 972 | WP_001103698.1 | hypothetical protein | - |
H4J01_RS24960 | 70482..71126 | + | 645 | WP_000633911.1 | AAA family ATPase | - |
H4J01_RS24965 | 71120..71395 | + | 276 | WP_000239529.1 | hypothetical protein | - |
H4J01_RS24970 | 71533..72342 | - | 810 | WP_000016979.1 | site-specific integrase | - |
H4J01_RS24975 | 72343..72648 | - | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
H4J01_RS24980 | 72650..72868 | - | 219 | WP_000813637.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
H4J01_RS24985 | 73582..76203 | + | 2622 | WP_000283686.1 | hypothetical protein | - |
H4J01_RS24990 | 76402..76632 | + | 231 | WP_001261274.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
H4J01_RS24995 | 76808..76996 | + | 189 | WP_001386137.1 | hypothetical protein | - |
H4J01_RS25000 | 77001..77218 | - | 218 | Protein_96 | transposase | - |
H4J01_RS25005 | 77219..77749 | - | 531 | WP_000348885.1 | GNAT family N-acetyltransferase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Conjugative plasmid | - | toxB / toxB / bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL | 1..96603 | 96603 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T169249 WP_001159871.1 NZ_CP059841:c72648-72343 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T169249 NZ_CP076318:c2518919-2518623 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8428.45 Da Isoelectric Point: 4.7405
>AT169249 WP_000813637.1 NZ_CP059841:c72868-72650 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKVENQEGMVEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKVENQEGMVEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT169249 NZ_CP076318:c2518621-2518226 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CCE8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | Q0H051 |