Detailed information of TA system TA169247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 11651..12186
• Replicon: plasmid pMAR2
• Accession: NZ_CP059841
• Organism: Escherichia coli strain E2348/69

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: H4J01_RS24595
• Protein ID: WP_000222766.1
• Coordinates: 11651..11938 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: H4J01_RS24600
• Protein ID: WP_001132900.1
• Coordinates: 11935..12186 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J01_RS24570	8640..9066	+	427	Protein_10	IS3 family transposase	-
H4J01_RS24575	9134..9570	-	437	Protein_11	ATP-binding protein	-
H4J01_RS24580	9583..10101	-	519	Protein_12	transposase	-
H4J01_RS24585	10188..11179	+	992	Protein_13	IS21 family transposase	-
H4J01_RS24590	11176..11445	+	270	Protein_14	transposase	-
H4J01_RS24595	11651..11938	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H4J01_RS24600	11935..12186	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H4J01_RS24605	12259..12450	-	192	WP_000841007.1	hypothetical protein	-
H4J01_RS24610	13151..14008	-	858	WP_000131012.1	incFII family plasmid replication initiator RepA	-
H4J01_RS24615	14001..14075	-	75	WP_001365705.1	RepA leader peptide Tap	-
H4J01_RS24620	14322..14570	-	249	WP_000083838.1	replication regulatory protein RepA	-
H4J01_RS24625	14854..15003	-	150	WP_001300273.1	type I toxin-antitoxin system Hok family toxin	-
H4J01_RS24630	15192..16565	+	1374	WP_000244358.1	IS4-like element ISEc13 family transposase	-
H4J01_RS24635	16598..17155	-	558	WP_000139319.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	toxB / toxB / bfpA / bfpG / bfpB / bfpC / bfpU / bfpD / bfpE / bfpF / bfpP / bfpH / bfpI / bfpJ / bfpK / bfpL	1..96603	96603
	-	inside	IScluster/Tn	-	toxB / toxB	1376..16565	15189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T169247 WP_000222766.1 NZ_CP059841:c11938-11651 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T169247 NZ_CP076318:c2310539-2310132 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT169247 WP_001132900.1 NZ_CP059841:c12186-11935 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT169247 NZ_CP076318:c2310786-2310520 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2