Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4108387..4108937 Replicon chromosome
Accession NZ_CP059840
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H4J01_RS20610 Protein ID WP_001160966.1
Coordinates 4108387..4108701 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H4J01_RS20615 Protein ID WP_000125566.1
Coordinates 4108704..4108937 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS20585 4104062..4105348 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H4J01_RS20590 4105348..4106337 + 990 WP_000241226.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H4J01_RS20595 4106334..4107155 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H4J01_RS20600 4107158..4107535 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H4J01_RS20605 4107542..4108390 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H4J01_RS20610 4108387..4108701 - 315 WP_001160966.1 CcdB family protein Toxin
H4J01_RS20615 4108704..4108937 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H4J01_RS20620 4109023..4109502 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H4J01_RS20625 4109694..4111556 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
H4J01_RS20630 4111549..4112079 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H4J01_RS20635 4112187..4113518 - 1332 WP_001183210.1 MFS transporter -
H4J01_RS20640 4113577..4113864 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T169244 WP_001160966.1 NZ_CP059840:c4108701-4108387 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T169244 NZ_CP076318:c1388061-1387807 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT169244 WP_000125566.1 NZ_CP059840:c4108937-4108704 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT169244 NZ_CP076318:c1388309-1388058 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References