Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2937038..2937822 Replicon chromosome
Accession NZ_CP059840
Organism Escherichia coli strain E2348/69

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag H4J01_RS14775 Protein ID WP_000613626.1
Coordinates 2937328..2937822 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag H4J01_RS14770 Protein ID WP_001110446.1
Coordinates 2937038..2937331 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J01_RS14760 2932187..2933146 - 960 WP_000846329.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
H4J01_RS14765 2933719..2936904 + 3186 WP_000827393.1 ribonuclease E -
H4J01_RS14770 2937038..2937331 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
H4J01_RS14775 2937328..2937822 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
H4J01_RS14780 2937917..2938870 - 954 WP_001212764.1 flagellar hook-associated protein FlgL -
H4J01_RS14785 2938882..2940525 - 1644 WP_000096508.1 flagellar hook-associated protein FlgK -
H4J01_RS14790 2940591..2941532 - 942 WP_012578881.1 flagellar assembly peptidoglycan hydrolase FlgJ -
H4J01_RS14795 2941532..2942629 - 1098 WP_000589300.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T169240 WP_000613626.1 NZ_CP059840:2937328-2937822 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T169240 NZ_CP076318:c565796-565689 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT169240 WP_001110446.1 NZ_CP059840:2937038-2937331 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT169240 NZ_CP076318:565843-565909 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References