Detailed information of TA system TA169238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2458306..2459894
• Replicon: chromosome
• Accession: NZ_CP059840
• Organism: Escherichia coli strain E2348/69

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7URP4
• Locus tag: H4J01_RS12295
• Protein ID: WP_001125430.1
• Coordinates: 2458572..2459894 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H4J01_RS12290
• Protein ID: WP_001334382.1
• Coordinates: 2458306..2458572 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J01_RS12275	2454675..2456126	+	1452	WP_000854637.1	tagaturonate reductase	-
H4J01_RS12280	2456323..2457237	+	915	WP_001313828.1	bestrophin family inner membrane protein	-
H4J01_RS12285	2457241..2457999	-	759	WP_001286539.1	trans-aconitate 2-methyltransferase	-
H4J01_RS12290	2458306..2458572	+	267	WP_001334382.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H4J01_RS12295	2458572..2459894	+	1323	WP_001125430.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
H4J01_RS12300	2460002..2460148	+	147	Protein_2409	site-specific integrase	-
H4J01_RS12305	2460140..2460391	+	252	WP_029694049.1	hypothetical protein	-
H4J01_RS12310	2460515..2460916	-	402	WP_012578908.1	reverse transcriptase	-
H4J01_RS12315	2461284..2461586	-	303	WP_000343124.1	hypothetical protein	-
H4J01_RS12320	2461913..2463739	-	1827	WP_012578907.1	AAA family ATPase	-
H4J01_RS12325	2463742..2464227	-	486	WP_001015823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2458306..2480297	21991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.96 Da
• Isoelectric Point: 8.1297

>T169238 WP_001125430.1 NZ_CP059840:2458572-2459894 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T169238 NZ_CP076318:c564726-564619 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10017.42 Da
• Isoelectric Point: 4.9632

>AT169238 WP_001334382.1 NZ_CP059840:2458306-2458572 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT169238 NZ_CP076318:564774-564840 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A0K4M2E2