Detailed information of TA system TA169219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 59420..59955
• Replicon: plasmid p0A3_5
• Accession: NZ_CP059839
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GQF93_RS27535
• Protein ID: WP_086589903.1
• Coordinates: 59420..59707 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: GQF93_RS27540
• Protein ID: WP_001132895.1
• Coordinates: 59704..59955 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS27515 (55906)	55906..56301	+	396	Protein_46	transposase	-
GQF93_RS27520 (56368)	56368..57107	+	740	Protein_47	tyrosine-type recombinase/integrase	-
GQF93_RS27525 (57386)	57386..58363	-	978	WP_086589904.1	RepB family plasmid replication initiator protein	-
GQF93_RS27530 (59007)	59007..59418	-	412	Protein_49	DDE-type integrase/transposase/recombinase	-
GQF93_RS27535 (59420)	59420..59707	-	288	WP_086589903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQF93_RS27540 (59704)	59704..59955	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GQF93_RS27545 (60129)	60129..60341	-	213	WP_000402944.1	hypothetical protein	-
GQF93_RS27550 (60715)	60715..61920	+	1206	WP_000817642.1	AAA family ATPase	-
GQF93_RS27555 (61917)	61917..62879	+	963	WP_000756325.1	ParB family protein	-
GQF93_RS27560 (63020)	63020..63193	-	174	Protein_55	DUF4113 domain-containing protein	-
GQF93_RS27565 (63267)	63267..63692	+	426	WP_024193300.1	antirestriction protein	-
GQF93_RS27570 (63729)	63729..64885	-	1157	Protein_57	RNA-guided endonuclease TnpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	etpB / etpB / etpA	1..82387	82387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.25 Da
• Isoelectric Point: 10.4703

>T169219 WP_086589903.1 NZ_CP059839:c59707-59420 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGLRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T169219 NZ_CP076305:c2695656-2695402 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT169219 WP_001132895.1 NZ_CP059839:c59955-59704 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT169219 NZ_CP076305:c2695904-2695653 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6