Detailed information of TA system TA169218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 37626..38148
• Replicon: plasmid pTET_A3_4
• Accession: NZ_CP059838
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: GQF93_RS27205
• Protein ID: WP_000220560.1
• Coordinates: 37867..38148 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GQF93_RS27200
• Protein ID: WP_212487696.1
• Coordinates: 37626..37877 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS27170 (GQF93_27160)	33246..33392	+	147	WP_251365475.1	hypothetical protein	-
GQF93_RS28170	33386..33841	+	456	WP_251365476.1	YcxB family protein	-
GQF93_RS27180 (GQF93_27170)	33893..34249	-	357	WP_000876433.1	DNA distortion polypeptide 3	-
GQF93_RS27185 (GQF93_27175)	34386..34832	-	447	WP_157701447.1	hypothetical protein	-
GQF93_RS27190 (GQF93_27180)	34836..35687	-	852	WP_212487694.1	replication initiation protein	-
GQF93_RS27195 (GQF93_27185)	35692..36561	-	870	WP_032347905.1	replication initiation protein	-
GQF93_RS27200 (GQF93_27190)	37626..37877	+	252	WP_212487696.1	plasmid stabilization protein	Antitoxin
GQF93_RS27205 (GQF93_27195)	37867..38148	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQF93_RS27210 (GQF93_27200)	38294..38617	+	324	WP_001181906.1	hypothetical protein	-
GQF93_RS27215 (GQF93_27205)	38665..38910	+	246	WP_000356542.1	hypothetical protein	-
GQF93_RS27220 (GQF93_27210)	38900..39115	+	216	WP_001180117.1	hypothetical protein	-
GQF93_RS27225 (GQF93_27215)	39208..39537	+	330	WP_212487698.1	hypothetical protein	-
GQF93_RS27230 (GQF93_27220)	39580..39759	+	180	WP_000439079.1	hypothetical protein	-
GQF93_RS27235 (GQF93_27225)	39829..39978	+	150	WP_212487700.1	hypothetical protein	-
GQF93_RS27240 (GQF93_27230)	39991..40263	+	273	WP_000160399.1	hypothetical protein	-
GQF93_RS27250 (GQF93_27240)	40589..41134	+	546	WP_000757692.1	DNA distortion polypeptide 1	-
GQF93_RS27255 (GQF93_27245)	41137..42303	+	1167	WP_000539539.1	MobP1 family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A)	-	1..51601	51601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T169218 WP_000220560.1 NZ_CP059838:37867-38148 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T169218 NZ_CP076305:c2476073-2475970 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.39 Da
• Isoelectric Point: 4.1225

>AT169218 WP_212487696.1 NZ_CP059838:37626-37877 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYQELAENAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT169218 NZ_CP076305:2475932-2476203 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: G3CAN5