Detailed information of TA system TA169213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 75364..75911
• Replicon: plasmid pCTX_A3_3
• Accession: NZ_CP059837
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GQF93_RS26680
• Protein ID: WP_244442846.1
• Coordinates: 75364..75642 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: GQF93_RS26685
• Protein ID: WP_000079941.1
• Coordinates: 75642..75911 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS26655 (71991)	71991..72593	+	603	WP_000517695.1	ProQ/FINO family protein	-
GQF93_RS26660 (72649)	72649..73143	+	495	WP_001459505.1	hypothetical protein	-
GQF93_RS26665 (73143)	73143..73409	+	267	WP_000955263.1	hypothetical protein	-
GQF93_RS26670 (73886)	73886..74359	-	474	WP_001334658.1	hypothetical protein	-
GQF93_RS26675 (74476)	74476..75324	-	849	WP_001057996.1	3'-5' exonuclease	-
GQF93_RS26680 (75364)	75364..75642	-	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
GQF93_RS26685 (75642)	75642..75911	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
GQF93_RS26690 (75966)	75966..76124	-	159	WP_000841008.1	hypothetical protein	-
GQF93_RS26695 (76820)	76820..77851	-	1032	WP_000907873.1	plasmid replication initiator RepA	-
GQF93_RS26700 (78809)	78809..79087	+	279	WP_219065241.1	ash family protein	-
GQF93_RS26705 (79005)	79005..79292	+	288	WP_000074849.1	conjugal transfer protein TraA	-
GQF93_RS26710 (79734)	79734..80267	+	534	WP_001303319.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia	-	1..133615	133615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T169213 WP_244442846.1 NZ_CP059837:c75642-75364 [Escherichia coli]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T169213 NZ_CP076305:274996-275169 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT169213 WP_000079941.1 NZ_CP059837:c75911-75642 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT169213 NZ_CP076305:274672-274968 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5