Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 39496..40139 Replicon plasmid pCTX_A3_3
Accession NZ_CP059837
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag GQF93_RS26490 Protein ID WP_001044768.1
Coordinates 39723..40139 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag GQF93_RS26485 Protein ID WP_001261287.1
Coordinates 39496..39726 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS26470 (35607) 35607..36695 + 1089 WP_000952231.1 hypothetical protein -
GQF93_RS26475 (36697) 36697..37566 + 870 WP_000253407.1 hypothetical protein -
GQF93_RS26480 (37623) 37623..39188 - 1566 WP_000741348.1 AAA family ATPase -
GQF93_RS26485 (39496) 39496..39726 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GQF93_RS26490 (39723) 39723..40139 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
GQF93_RS26495 (40301) 40301..42439 - 2139 WP_000350635.1 AAA family ATPase -
GQF93_RS26500 (42793) 42793..43050 + 258 WP_000343085.1 hypothetical protein -
GQF93_RS26505 (43050) 43050..43640 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / fosA3 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia - 1..133615 133615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T169212 WP_001044768.1 NZ_CP059837:39723-40139 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T169212 NZ_CP076304:c43630-43478 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT169212 WP_001261287.1 NZ_CP059837:39496-39726 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT169212 NZ_CP076304:43682-43742 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References