Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 8724..10347 Replicon plasmid pMCR_A3_2
Accession NZ_CP059836
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag GQF93_RS24855 Protein ID WP_000088046.1
Coordinates 9022..10347 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag GQF93_RS24850 Protein ID WP_024136327.1
Coordinates 8724..9017 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS24825 (GQF93_24820) 4596..5837 + 1242 WP_001053338.1 VWA domain-containing protein -
GQF93_RS28320 5928..6059 + 132 WP_000374059.1 hypothetical protein -
GQF93_RS24830 (GQF93_24825) 6400..6681 - 282 WP_000077926.1 hypothetical protein -
GQF93_RS24835 (GQF93_24830) 6731..6922 - 192 WP_000398480.1 hypothetical protein -
GQF93_RS24840 (GQF93_24835) 7014..7355 - 342 WP_001151575.1 hypothetical protein -
GQF93_RS24845 (GQF93_24840) 7728..8120 + 393 WP_000341066.1 hypothetical protein -
GQF93_RS24850 (GQF93_24845) 8724..9017 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
GQF93_RS24855 (GQF93_24850) 9022..10347 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
GQF93_RS24860 (GQF93_24855) 10408..10614 + 207 WP_000134171.1 hypothetical protein -
GQF93_RS24865 (GQF93_24860) 10716..11126 + 411 WP_000985911.1 hypothetical protein -
GQF93_RS24870 (GQF93_24865) 11139..11954 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
GQF93_RS24875 (GQF93_24870) 12208..12633 + 426 WP_001043843.1 hypothetical protein -
GQF93_RS24880 (GQF93_24875) 13182..13490 + 309 WP_001224687.1 hypothetical protein -
GQF93_RS24885 (GQF93_24880) 13506..14363 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / tet(A) / tet(M) / qnrS2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / aph(3')-Ia / oqxA / oqxB / mcr-1.1 - 1..267184 267184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T169211 WP_000088046.1 NZ_CP059836:9022-10347 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T169211 NZ_CP076303:79953-80333 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT169211 WP_024136327.1 NZ_CP059836:8724-9017 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT169211 NZ_CP076303:79732-79953 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References