Detailed information of TA system TA169204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4256153..4256778
• Replicon: chromosome
• Accession: NZ_CP059835
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GQF93_RS20725
• Protein ID: WP_000911330.1
• Coordinates: 4256380..4256778 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GQF93_RS20720
• Protein ID: WP_000450524.1
• Coordinates: 4256153..4256380 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS20695 (4251956)	4251956..4252426	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GQF93_RS20700 (4252426)	4252426..4252998	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GQF93_RS20705 (4253144)	4253144..4254022	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GQF93_RS20710 (4254039)	4254039..4255073	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
GQF93_RS20715 (4255286)	4255286..4255999	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GQF93_RS20720 (4256153)	4256153..4256380	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GQF93_RS20725 (4256380)	4256380..4256778	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
GQF93_RS20730 (4256925)	4256925..4257788	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
GQF93_RS20735 (4257803)	4257803..4259818	+	2016	WP_097432819.1	tRNA cytosine(34) acetyltransferase TmcA	-
GQF93_RS20740 (4259892)	4259892..4260590	+	699	WP_000679823.1	esterase	-
GQF93_RS20745 (4260700)	4260700..4260900	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU	4117137..4284972	167835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T169204 WP_000911330.1 NZ_CP059835:4256380-4256778 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T169204 NZ_CP076301:4381874-4382272 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT169204 WP_000450524.1 NZ_CP059835:4256153-4256380 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT169204 NZ_CP076301:4381578-4381871 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33