Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3721618..3722272 Replicon chromosome
Accession NZ_CP059835
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GQF93_RS18005 Protein ID WP_000244772.1
Coordinates 3721865..3722272 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GQF93_RS18000 Protein ID WP_000354046.1
Coordinates 3721618..3721884 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS17975 (3716787) 3716787..3717530 + 744 WP_097432787.1 SDR family oxidoreductase -
GQF93_RS17980 (3717587) 3717587..3719020 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
GQF93_RS17985 (3719065) 3719065..3719376 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GQF93_RS17990 (3719540) 3719540..3720199 + 660 WP_000250274.1 hemolysin III family protein -
GQF93_RS17995 (3720395) 3720395..3721375 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GQF93_RS18000 (3721618) 3721618..3721884 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GQF93_RS18005 (3721865) 3721865..3722272 + 408 WP_000244772.1 protein YgfX Toxin
GQF93_RS18010 (3722312) 3722312..3722833 - 522 WP_001055874.1 flavodoxin FldB -
GQF93_RS18015 (3722945) 3722945..3723841 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GQF93_RS18020 (3723866) 3723866..3724576 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GQF93_RS18025 (3724582) 3724582..3726315 + 1734 WP_212487191.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T169202 WP_000244772.1 NZ_CP059835:3721865-3722272 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T169202 NZ_CP076301:3885327-3885617 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT169202 WP_000354046.1 NZ_CP059835:3721618-3721884 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT169202 NZ_CP076301:3885095-3885337 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References