Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2738672..2739258 Replicon chromosome
Accession NZ_CP059835
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag GQF93_RS13450 Protein ID WP_042014162.1
Coordinates 2739028..2739258 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GQF93_RS13445 Protein ID WP_000593555.1
Coordinates 2738672..2739031 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS13420 (2734804) 2734804..2735748 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
GQF93_RS13425 (2735745) 2735745..2736578 + 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
GQF93_RS13430 (2736578) 2736578..2737342 + 765 WP_024243155.1 nickel import ATP-binding protein NikD -
GQF93_RS13435 (2737339) 2737339..2738145 + 807 WP_000173630.1 nickel import ATP-binding protein NikE -
GQF93_RS13440 (2738151) 2738151..2738552 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
GQF93_RS13445 (2738672) 2738672..2739031 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GQF93_RS13450 (2739028) 2739028..2739258 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
GQF93_RS13455 (2739362) 2739362..2740486 - 1125 WP_001216257.1 ABC transporter permease -
GQF93_RS13460 (2740486) 2740486..2743221 - 2736 WP_024243304.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T169198 WP_042014162.1 NZ_CP059835:c2739258-2739028 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T169198 NZ_CP076301:3429728-3430039 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT169198 WP_000593555.1 NZ_CP059835:c2739031-2738672 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT169198 NZ_CP076301:3430040-3430330 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References