Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1463291..1464128 Replicon chromosome
Accession NZ_CP059835
Organism Escherichia coli strain tcmA_3

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GQF93_RS07215 Protein ID WP_000227784.1
Coordinates 1463586..1464128 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GQF93_RS07210 Protein ID WP_001297137.1
Coordinates 1463291..1463602 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF93_RS07185 (1458311) 1458311..1459258 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
GQF93_RS07190 (1459280) 1459280..1461271 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GQF93_RS07195 (1461261) 1461261..1461875 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GQF93_RS07200 (1461875) 1461875..1462204 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GQF93_RS07205 (1462216) 1462216..1463106 + 891 WP_000971336.1 heme o synthase -
GQF93_RS07210 (1463291) 1463291..1463602 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GQF93_RS07215 (1463586) 1463586..1464128 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GQF93_RS07220 (1464184) 1464184..1465119 - 936 WP_001297127.1 tetratricopeptide repeat protein -
GQF93_RS07225 (1465527) 1465527..1466891 + 1365 WP_001000978.1 MFS transporter -
GQF93_RS07230 (1467019) 1467019..1467510 - 492 WP_001138904.1 nucleotide binding protein YajQ -
GQF93_RS07235 (1467678) 1467678..1468589 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T169193 WP_000227784.1 NZ_CP059835:1463586-1464128 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T169193 NZ_CP076301:c2505502-2505206 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT169193 WP_001297137.1 NZ_CP059835:1463291-1463602 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT169193 NZ_CP076301:c2505204-2504809 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References