Detailed information of TA system TA169182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 184181..184819
• Replicon: chromosome
• Accession: NZ_CP059835
• Organism: Escherichia coli strain tcmA_3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GQF93_RS00890
• Protein ID: WP_000813794.1
• Coordinates: 184181..184357 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GQF93_RS00895
• Protein ID: WP_001270286.1
• Coordinates: 184403..184819 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF93_RS00870 (179800)	179800..181014	-	1215	WP_001349372.1	BenE family transporter YdcO	-
GQF93_RS00875 (181067)	181067..181603	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
GQF93_RS00880 (181676)	181676..183637	+	1962	WP_001542890.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GQF93_RS00885 (183729)	183729..183959	-	231	WP_000494244.1	YncJ family protein	-
GQF93_RS00890 (184181)	184181..184357	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GQF93_RS00895 (184403)	184403..184819	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GQF93_RS00900 (184898)	184898..186136	+	1239	Protein_184	PLP-dependent aminotransferase family protein	-
GQF93_RS00905 (186222)	186222..187214	+	993	WP_251365460.1	PLP-dependent aminotransferase family protein	-
GQF93_RS00910 (187459)	187459..188604	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
GQF93_RS00915 (188622)	188622..189635	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	178612..179760	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T169182 WP_000813794.1 NZ_CP059835:184181-184357 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T169182 NZ_CP076301:312276-312379 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT169182 WP_001270286.1 NZ_CP059835:184403-184819 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT169182 NZ_CP076301:c312417-312146 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• PDB: 6HPB