Detailed information of TA system TA169180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 150886..152023
• Replicon: plasmid pA15023_P1
• Accession: NZ_CP059824
• Organism: Enterococcus faecium strain A15023

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HI843_RS14385
• Protein ID: WP_002401483.1
• Coordinates: 150886..151749 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI843_RS14390
• Protein ID: WP_000301765.1
• Coordinates: 151751..152023 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI843_RS14355	146779..148269	-	1491	WP_002380753.1	primase C-terminal domain-containing protein	-
HI843_RS14360	148664..148903	-	240	WP_002380754.1	helix-turn-helix domain-containing protein	-
HI843_RS14365	149147..149320	-	174	WP_171902975.1	rRNA adenine methyltransferase	-
HI843_RS14370	149265..150002	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI843_RS14375	150127..150210	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
HI843_RS14380	150259..150342	-	84	Protein_155	peptide-binding protein	-
HI843_RS14385	150886..151749	-	864	WP_002401483.1	zeta toxin family protein	Toxin
HI843_RS14390	151751..152023	-	273	WP_000301765.1	antitoxin	Antitoxin
HI843_RS14395	152040..152249	-	210	WP_000527317.1	peptide-binding protein	-
HI843_RS14400	152326..153222	-	897	WP_118215883.1	ParA family protein	-
HI843_RS14405	153325..153585	-	261	Protein_160	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
HI843_RS14410	153755..154492	-	738	WP_182481801.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI843_RS14415	154617..154712	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
HI843_RS14420	154761..155000	-	240	WP_000635249.1	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / erm(B)	-	1..206393	206393
	-	inside	IScluster/Tn	aac(6')-aph(2'') / erm(B)	-	142309..161202	18893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T169180 WP_002401483.1 NZ_CP059824:c151749-150886 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T169180 NZ_CP059824:c151749-150886 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GATTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT169180 WP_000301765.1 NZ_CP059824:c152023-151751 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT169180 NZ_CP059824:c152023-151751 [Enterococcus faecium]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3