Detailed information of TA system TA169179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 478464..479035
• Replicon: chromosome
• Accession: NZ_CP059823
• Organism: Enterococcus faecium strain A15023

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HI843_RS02275
• Protein ID: WP_169045386.1
• Coordinates: 478694..479035 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: HI843_RS02270
• Protein ID: WP_002323011.1
• Coordinates: 478464..478694 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI843_RS02240	473600..474928	+	1329	WP_008265691.1	FAD-containing oxidoreductase	-
HI843_RS02245	474950..475576	+	627	WP_002286816.1	cysteine hydrolase	-
HI843_RS02250	475759..476340	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
HI843_RS02255	477072..477419	+	348	WP_002286808.1	SOS response-associated peptidase family protein	-
HI843_RS02260	477395..477634	+	240	WP_002321938.1	SOS response-associated peptidase family protein	-
HI843_RS02265	477839..478177	-	339	WP_002286804.1	hypothetical protein	-
HI843_RS02270	478464..478694	+	231	WP_002323011.1	hypothetical protein	Antitoxin
HI843_RS02275	478694..479035	+	342	WP_169045386.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HI843_RS02280	479885..480070	+	186	WP_002304207.1	hypothetical protein	-
HI843_RS02285	480575..480769	+	195	WP_002297028.1	hypothetical protein	-
HI843_RS02290	480927..481209	+	283	Protein_454	transposase	-
HI843_RS02295	481453..482283	-	831	WP_002286789.1	manganese catalase family protein	-
HI843_RS02300	482491..483825	+	1335	WP_002286788.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13268.66 Da
• Isoelectric Point: 9.9044

>T169179 WP_169045386.1 NZ_CP059823:478694-479035 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDLKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T169179 NZ_CP076298:c3084404-3084183 [Burkholderia pseudomallei]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT169179 WP_002323011.1 NZ_CP059823:478464-478694 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT169179 NZ_CP076298:c3084199-3083915 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9