169177

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 13251..14388 Replicon plasmid pBP657_P3
Accession NZ_CP059819
Organism Enterococcus faecium strain BP657

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag HI829_RS15095 Protein ID WP_001284311.1
Coordinates 13525..14388 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag HI829_RS15090 Protein ID WP_000301765.1
Coordinates 13251..13523 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI829_RS15050 8363..8659 + 297 WP_000053907.1 replication control protein PrgN -
HI829_RS15055 8683..9642 - 960 WP_002287225.1 IS30 family transposase -
HI829_RS15060 9914..10594 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HI829_RS15065 10669..11286 - 618 Protein_13 tetracycline resistance ribosomal protection protein -
HI829_RS15070 11377..12057 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
HI829_RS15075 12114..12614 + 501 Protein_15 IS6-like element IS1216 family transposase -
HI829_RS15080 12670..12927 + 258 WP_002347171.1 hypothetical protein -
HI829_RS15085 13025..13234 + 210 WP_000527318.1 peptide-binding protein -
HI829_RS15090 13251..13523 + 273 WP_000301765.1 antitoxin Antitoxin
HI829_RS15095 13525..14388 + 864 WP_001284311.1 toxin zeta Toxin
HI829_RS15100 14651..15388 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
HI829_RS15105 15513..15596 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
HI829_RS15110 15645..15728 - 84 Protein_22 peptide-binding protein -
HI829_RS15115 15885..16844 - 960 WP_002303350.1 IS30 family transposase -
HI829_RS15120 17204..17998 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
HI829_RS15125 18091..18342 - 252 Protein_25 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III - 1..24162 24162
- inside IScluster/Tn erm(B) / aph(3')-III - 51..20628 20577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T169177 WP_001284311.1 NZ_CP059819:13525-14388 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T169177 NZ_CP076298:1656764-1657033 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATTATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT169177 WP_000301765.1 NZ_CP059819:13251-13523 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT169177 NZ_CP076298:1657017-1657301 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGACCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGTAAGCGCGGTCGTCCACCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGAGAAGGCTGGCAGACGCGCATG
AACGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References