169173

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 482600..483171 Replicon chromosome
Accession NZ_CP059816
Organism Enterococcus faecium strain BP657

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag HI829_RS02280 Protein ID WP_002286801.1
Coordinates 482830..483171 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag HI829_RS02275 Protein ID WP_002323011.1
Coordinates 482600..482830 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI829_RS02245 477859..479187 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
HI829_RS02250 479209..479835 + 627 WP_002286816.1 cysteine hydrolase -
HI829_RS02255 480018..480599 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
HI829_RS02260 481208..481555 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
HI829_RS02265 481531..481770 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
HI829_RS02270 481975..482313 - 339 WP_002286804.1 hypothetical protein -
HI829_RS02275 482600..482830 + 231 WP_002323011.1 hypothetical protein Antitoxin
HI829_RS02280 482830..483171 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HI829_RS02285 484021..484206 + 186 WP_002304207.1 hypothetical protein -
HI829_RS02290 484711..484905 + 195 WP_002297028.1 hypothetical protein -
HI829_RS02295 485063..485345 + 283 Protein_455 transposase -
HI829_RS02300 485589..486419 - 831 WP_002286789.1 manganese catalase family protein -
HI829_RS02305 486627..487961 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T169173 WP_002286801.1 NZ_CP059816:482830-483171 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T169173 NZ_CP076291:488783-489133 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT169173 WP_002323011.1 NZ_CP059816:482600-482830 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT169173 NZ_CP076291:488496-488777 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References